Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line|
|Citation:||Obesity Research & Clinical Practice, 2011; 5(1):43-54|
|Yan Y. Lam, Alena Janovská, Andrew J. McAinch, Damien P. Belobrajdic, George Hatzinikolas, Philip Game, Gary A. Wittert|
|Abstract:||<h4>Summary</h4>We aimed to study the depot-specific effect of adipose tissue on insulin sensitivity of skeletal muscle in vitro. Adipose tissue-conditioned medium (CM) was generated from visceral and subcutaneous fat from obese subjects. CM from visceral as compared to subcutaneous fat had higher concentrations of interleukin (IL)-6 (15-fold; P < 0.05) and IL-8 (8-fold; P < 0.05). CM from visceral fat (1:128 dilution) reduced insulin-stimulated glucose uptake in L6 myotubes by 19% (P < 0.05), an effect mediated by a nuclear factor kappa B (NFκB)/mammalian target of rapamycin complex 1 (mTORC1)-dependent pathway and partially reversed by neutralizing IL-6. IL-6 at a concentration comparable to that in CM from visceral fat reduced insulin-stimulated glucose uptake by 53% (P < 0.05), an effect abolished by inhibiting NFκB or mTORC1. We demonstrated the utility of the CM-myotube system and identified IL-6 as a major cytokine mediating visceral fat-induced muscle insulin resistance.:|
|Keywords:||Adipokines; Skeletal muscle; Insulin resistance|
|Rights:||Copyright © 2010 Asian Oceanian Association for the Study of Obesity.|
|Appears in Collections:||Medicine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.