Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/68870
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Type: Journal article
Title: The use of adipose tissue-conditioned media to demonstrate the differential effects of fat depots on insulin-stimulated glucose uptake in a skeletal muscle cell line
Author: Lam, Y.
Janovska, A.
McAinch, A.
Belobrajdic, D.
Hatzinikolas, G.
Game, P.
Wittert, G.
Citation: Obesity Research & Clinical Practice, 2011; 5(1):43-54
Publisher: Elsevier BV
Issue Date: 2011
ISSN: 1871-403X
Statement of
Responsibility: 
Yan Y. Lam, Alena Janovská, Andrew J. McAinch, Damien P. Belobrajdic, George Hatzinikolas, Philip Game, Gary A. Wittert
Abstract: <h4>Summary</h4>We aimed to study the depot-specific effect of adipose tissue on insulin sensitivity of skeletal muscle in vitro. Adipose tissue-conditioned medium (CM) was generated from visceral and subcutaneous fat from obese subjects. CM from visceral as compared to subcutaneous fat had higher concentrations of interleukin (IL)-6 (15-fold; P < 0.05) and IL-8 (8-fold; P < 0.05). CM from visceral fat (1:128 dilution) reduced insulin-stimulated glucose uptake in L6 myotubes by 19% (P < 0.05), an effect mediated by a nuclear factor kappa B (NFκB)/mammalian target of rapamycin complex 1 (mTORC1)-dependent pathway and partially reversed by neutralizing IL-6. IL-6 at a concentration comparable to that in CM from visceral fat reduced insulin-stimulated glucose uptake by 53% (P < 0.05), an effect abolished by inhibiting NFκB or mTORC1. We demonstrated the utility of the CM-myotube system and identified IL-6 as a major cytokine mediating visceral fat-induced muscle insulin resistance.:
Keywords: Adipokines; Skeletal muscle; Insulin resistance
Rights: Copyright © 2010 Asian Oceanian Association for the Study of Obesity.
RMID: 0020110982
DOI: 10.1016/j.orcp.2010.12.002
Appears in Collections:Medicine publications

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