Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/6898
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: A randomized trial of supplementation with docosahexaenoic acid-rich tuna oil and its effects on the human milk cytokines interleukin 1beta, interleukin 6, and tumor necrosis factor alpha
Author: Hawkes, J.
Bryan, D.
Makrides, M.
Neumann, M.
Gibson, R.
Citation: American Journal of Clinical Nutrition, 2002; 75(4):754-760
Publisher: Amer Soc Clinical Nutrition
Issue Date: 2002
ISSN: 0002-9165
1938-3207
Statement of
Responsibility: 
Joanna S Hawkes, Dani-Louise Bryan, Maria Makrides, Mark A Neumann, and Robert A Gibson
Abstract: Background: Increased consumption of n-3 long-chain polyunsaturated fatty acids (PUFAs) has been recommended during pregnancy and lactation. The production of proinflammatory cytokines by human peripheral blood mononuclear cells (PBMCs) can be modified by dietary n-3 PUFAs. Objective: We sought to determine whether dietary supplementation of lactating women with docosahexaenoic acid (DHA) can modulate the concentration of cytokines in the aqueous phase of human milk and the production of cytokines by human milk cells (HMCs) and PBMCs. Design: In this double-blind, prospective, randomized trial, mothers of healthy full-term infants were asked to consume daily a nutritional supplement of 2000 mg oil containing either placebo (n = 40), 300 mg DHA + 70 mg eicosapentaenoic acid (EPA; n = 40), or 600 mg DHA + 140 mg EPA (n = 40). The fatty acid composition of plasma, PBMCs, milk, and HMCs from lactating mothers at 4 wk postpartum was measured by gas chromatography. The concentration of interleukin 6 and tumor necrosis factor in milk and the production of interleukin 1ß, tumor necrosis factor , and interleukin 6 by PBMCs and HMCs after stimulation with lipopolysaccharide was measured by enzyme-linked immunosorbent assay. Results: At 4 wk postpartum, 82 mothers were still breast-feeding their infants. DHA increases in maternal plasma, PBMCs, milk, and HMCs were proportional to dietary DHA. There was no relation between tissue DHA status and cytokine concentrations. Conclusions: Consumption of 600 mg DHA and 140 mg EPA/d for 4 wk increased n-3 PUFA concentrations in relevant tissues but did not cause perturbations in cytokine concentrations in human milk.
Keywords: Docosahexaenoic acid; human milk; cytokines; n-3 fatty acids; human milk cells; interleukin 1β; interleukin 6; tumor necrosis factor α; randomized controlled trial; breast-feeding; women
RMID: 0020020044
DOI: 10.1093/ajcn/75.4.754
Published version: http://www.ajcn.org/cgi/content/full/75/4/754
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.