Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/69005
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Type: Journal article
Title: Naloxone-precipitated morphine withdrawal behavior and brain IL-1β expression: Comparison of different mouse strains
Other Titles: Naloxone-precipitated morphine withdrawal behavior and brain IL-1beta expression: Comparison of different mouse strains
Author: Liu, L.
Coller, J.
Watkins, L.
Somogyi, A.
Hutchinson, M.
Citation: Brain Behavior and Immunity, 2011; 25(6):1223-1232
Publisher: Academic Press Inc
Issue Date: 2011
ISSN: 0889-1591
1090-2139
Statement of
Responsibility: 
Liang Liu, Janet K. Coller, Linda R. Watkins, Andrew A. Somogyi, Mark R. Hutchinson
Abstract: The development of opioid dependence involves classical neuronal opioid receptor activation and is due in part to engagement of glia causing a proinflammatory response. Such opioid-induced glial activation occurs, at least in part, through a non-classical opioid mechanism involving Toll-like-receptor 4 (TLR4). Among the immune factors released following the opioid-glia-TLR4 interaction, interleukin-1β (IL-1β) plays a prominent role. Previous animal behavioral studies have demonstrated significant heterogeneity of chronic morphine-induced tolerance and dependence between different mouse strains. The aim of this study was to investigate whether the heterogeneity of chronic opioid-induced IL-1β expression contributes to differences in opioid tolerance and withdrawal behaviors. Chronic morphine-induced tolerance and dependence were assessed in 3 inbred wild-type mouse strains (Balb/c, CBA, and C57BL/6) and 2 knockout strains (TLR4 and MyD88). Analysis of brain nuclei (medial prefrontal cortex, cortex, brain stem, hippocampus, and midbrain and diencephalon regions combined) revealed that, of inbred wild-type mice, there are significant main effects of morphine treatment on IL-1β expression in the brain regions analyzed (p<0.02 for all regions analyzed). A significant increase in hippocampal IL-1β expression was found in C57BL/6 mice after morphine treatment, whilst, a significant decrease was found in the mPFC region of wild-type Balb/c mice. Furthermore, the results of wild-type inbred strains demonstrated that the elevated hippocampal IL-1β expression is associated with withdrawal jumping behavior. Interestingly, knockout of TLR4, but not MyD88 protected against the development of analgesic tolerance. Gene sequence differences of IL - 1β and TLR4 genes alone did not explain the heterogeneity of dependence behavior between mouse strains. Together, these data further support the involvement of opioid-induced CNS immune signaling in dependence development. Moreover, this study demonstrated the advantages of utilizing multiple mouse strains and indicates that appropriate choice of mouse strains could enhance future research outcomes.
Keywords: Morphine; Glia; Interleukin-1β; Toll-like receptor 4; MyD88; Tolerance; Withdrawal; Mice strain differences
Rights: Copyright © 2011 Elsevier Inc. All rights reserved.
RMID: 0020111550
DOI: 10.1016/j.bbi.2011.03.016
Grant ID: http://purl.org/au-research/grants/nhmrc/465423
Appears in Collections:Pharmacology publications

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