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Type: Journal article
Title: Assessing the role of root plasma membrane and tonoplast Na⁺/H⁺ exchangers in salinity tolerance in wheat: in planta quantification methods
Other Titles: Assessing the role of root plasma membrane and tonoplast Na(+)/H(+) exchangers in salinity tolerance in wheat: in planta quantification methods
Author: Cuin, Tracey A.
Bose, Jayakumar
Stefano, Giovanni
Jha, Deepa Kumari
Tester, Mark Alfred
Mancuso, Stefano
Shabala, Sergey
Citation: Plant Cell and Environment, 2011; 34(6):947-961
Publisher: Blackwell Publishing Ltd
Issue Date: 2011
ISSN: 0140-7791
School/Discipline: School of Agriculture, Food and Wine : Plant and Food Science
Statement of
Tracey A. Cuin, Jayakumar Bose, Giovanni Stefano, Deepa Jha, Mark Tester, Stefano Mancuso & Sergey Shabala
Abstract: This work investigates the role of cytosolic Na⁺ exclusion in roots as a means of salinity tolerance in wheat, and offers in planta methods for the functional assessment of major transporters contributing to this trait. An electrophysiological protocol was developed to quantify the activity of plasma membrane Na⁺ efflux systems in roots, using the microelectrode ion flux estimation (MIFE) technique. We show that active efflux of Na⁺ from wheat root epidermal cells is mediated by a SOS1-like homolog, energized by the plasma membrane H⁺-ATPase. SOS1-like efflux activity was highest in Kharchia 65, a salt-tolerant bread wheat cultivar. Kharchia 65 also had an enhanced ability to sequester large quantities of Na⁺ into the vacuoles of root cells, as revealed by confocal microscopy using Sodium Green. These findings were consistent with the highest level of expression of both SOS1 and NHX1 transcripts in plant roots in this variety. In the sensitive wheat varieties, a greater proportion of Na⁺ was located in the root cell cytosol. Overall, our findings suggest a critical role of cytosolic Na⁺ exclusion for salinity tolerance in wheat and offer convenient protocols to quantify the contribution of the major transporters conferring this trait, to screen plants for salinity tolerance.
Keywords: cytosol; salinity tolerance; sodium; vacuolar sequestration.
Rights: © 2011 Blackwell Publishing Ltd.
DOI: 10.1111/j.1365-3040.2011.02296.x
Appears in Collections:Agriculture, Food and Wine publications

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