Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/69936
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dc.contributor.authorBoakye, P.en
dc.contributor.authorStenkamp-Strahm, C.en
dc.contributor.authorBhattarai, Y.en
dc.contributor.authorHeckman, M.en
dc.contributor.authorBrierley, S.en
dc.contributor.authorPasilis, S.en
dc.contributor.authorBalemba, O.en
dc.date.issued2012en
dc.identifier.citationNeurogastroenterology and Motility, 2012; 24(1):E27-E40en
dc.identifier.issn1350-1925en
dc.identifier.issn1365-2982en
dc.identifier.urihttp://hdl.handle.net/2440/69936-
dc.description.abstractBackground: Garcinia buchananii bark extract is an anti-motility diarrhea remedy. We investigated whether G. buchananii bark extract has components that reduce gastrointestinal peristaltic activity via 5-HT3 and 5-HT4 receptors. Methods:  Aqueous G. buchananii extract was separated into fractions using preparative thin layer chromatography (PTLC), and major chemical components were identified using standard tests. The anti-motility effects of the extract and its fractions (PTLC1-5) were studied through pellet propulsion assays using isolated guinea-pig distal colons. Key Results:  Anti-motility (PTLC1 & PTLC5) and pro-motility (PTLC2) fractions were isolated from the extract. Flavonoids, steroids, alkaloids, tannins, and phenols were identified in the extract and PTLC1&5. The potency of the extract applied via the mucosal surface was reduced by 5-HT, 5-HT3 receptor agonist RS-56812, 5-HT4 receptor agonists cisapride and CJ-033466, 5-HT3 receptor antagonist granisetron, and 5-HT4 receptor antagonist GR-113808. The anti-motility effects of the aqueous extract and PTLC1&5 when applied serosally were reversed by RS-56812, cisapride, and CJ-033466. The 5-HT3 receptor antagonists, granisetron and ondansetron, reduced the effects of the extract to an extent and completely reversed the anti-motility effects of PTLC1&5. GR-113808 inhibited the actions of the extract during the initial 10 min, but enhanced the extracts’ anti-motility effects after 15 min. GR-113808 augmented the anti-motility activities of PTLC1 and PTLC5 by 30%. Conclusions & Inferences:  These results indicate that the anti-motility effects of G. buchananii aqueous extract are potentially mediated by compounds that affect 5-HT3 and 5-HT4 receptors. Identification and characterization of the bioactive compounds within G. buchananii could lead to the discovery of new non-opiate anti-diarrhea formulations.en
dc.description.statementofresponsibilityP. A. Boakye, C. Stenkamp-Strahm, Y. Bhattarai, M. D. Heckman, S. M. Brierley, S. P. Pasilis, & O. B. Balembaen
dc.language.isoenen
dc.publisherBlackwell Publishing Ltden
dc.rights© 2011 Blackwell Publishing Ltd.en
dc.subjectdiarrhea; Garcinia; herbal remedy; intestinal motility; serotonin and 5-hydroxytryptamine.en
dc.title5-HT₃ and 5-HT₄ receptors contribute to the anti-motility effects of Garcinia buchananii bark extract in the guinea-pig distal colonen
dc.title.alternative5-HT(3) and 5-HT(4) receptors contribute to the anti-motility effects of Garcinia buchananii bark extract in the guinea-pig distal colonen
dc.typeJournal articleen
dc.identifier.rmid0020116073en
dc.identifier.doi10.1111/j.1365-2982.2011.01807.xen
dc.identifier.pubid26133-
pubs.library.collectionMedicine publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidBrierley, S. [0000-0002-2527-2905]en
Appears in Collections:Medicine publications

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