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Type: Journal article
Title: 5-HT₃ and 5-HT₄ receptors contribute to the anti-motility effects of Garcinia buchananii bark extract in the guinea-pig distal colon
Other Titles: 5-HT(3) and 5-HT(4) receptors contribute to the anti-motility effects of Garcinia buchananii bark extract in the guinea-pig distal colon
Author: Boakye, P.
Stenkamp-Strahm, C.
Bhattarai, Y.
Heckman, M.
Brierley, S.
Pasilis, S.
Balemba, O.
Citation: Neurogastroenterology and Motility, 2012; 24(1):E27-E40
Publisher: Blackwell Publishing Ltd
Issue Date: 2012
ISSN: 1350-1925
Statement of
P. A. Boakye, C. Stenkamp-Strahm, Y. Bhattarai, M. D. Heckman, S. M. Brierley, S. P. Pasilis, & O. B. Balemba
Abstract: Background: Garcinia buchananii bark extract is an anti-motility diarrhea remedy. We investigated whether G. buchananii bark extract has components that reduce gastrointestinal peristaltic activity via 5-HT3 and 5-HT4 receptors. Methods:  Aqueous G. buchananii extract was separated into fractions using preparative thin layer chromatography (PTLC), and major chemical components were identified using standard tests. The anti-motility effects of the extract and its fractions (PTLC1-5) were studied through pellet propulsion assays using isolated guinea-pig distal colons. Key Results:  Anti-motility (PTLC1 & PTLC5) and pro-motility (PTLC2) fractions were isolated from the extract. Flavonoids, steroids, alkaloids, tannins, and phenols were identified in the extract and PTLC1&5. The potency of the extract applied via the mucosal surface was reduced by 5-HT, 5-HT3 receptor agonist RS-56812, 5-HT4 receptor agonists cisapride and CJ-033466, 5-HT3 receptor antagonist granisetron, and 5-HT4 receptor antagonist GR-113808. The anti-motility effects of the aqueous extract and PTLC1&5 when applied serosally were reversed by RS-56812, cisapride, and CJ-033466. The 5-HT3 receptor antagonists, granisetron and ondansetron, reduced the effects of the extract to an extent and completely reversed the anti-motility effects of PTLC1&5. GR-113808 inhibited the actions of the extract during the initial 10 min, but enhanced the extracts’ anti-motility effects after 15 min. GR-113808 augmented the anti-motility activities of PTLC1 and PTLC5 by 30%. Conclusions & Inferences:  These results indicate that the anti-motility effects of G. buchananii aqueous extract are potentially mediated by compounds that affect 5-HT3 and 5-HT4 receptors. Identification and characterization of the bioactive compounds within G. buchananii could lead to the discovery of new non-opiate anti-diarrhea formulations.
Keywords: diarrhea; Garcinia; herbal remedy; intestinal motility; serotonin and 5-hydroxytryptamine.
Rights: © 2011 Blackwell Publishing Ltd.
RMID: 0020116073
DOI: 10.1111/j.1365-2982.2011.01807.x
Appears in Collections:Medicine publications

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