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Type: Journal article
Title: Molecular cloning and chromosomal mapping of a novel five-span transmembrane protein gene, M83
Author: Motohashi, T.
Miyoshi, S.
Osawa, M.
Eyre, H.
Sutherland, G.
Matsuda, Y.
Nakamura, Y.
Shibuya, A.
Iwama, A.
Nakauchi, H.
Citation: Biochemical and Biophysical Research Communications, 2000; 276(1):244-250
Publisher: Academic Press Inc
Issue Date: 2000
ISSN: 0006-291X
Statement of
Tsutomu Motohashi, Sousuke Miyoshi, Mitsujiro Osawa, Helen J. Eyre, Grant R. Sutherland, Yoichi Matsuda, Yukio Nakamura, Akira Shibuya, Atsushi Iwama, and Hiromitsu Nakauchi
Abstract: In an attempt to identify novel transmembrane molecules expressed on hematopoietic cells, we identified a novel transmembrane protein gene, M83. Cloning of the full-length cDNAs of human and mouse M83 revealed that M83 encodes a type I transmembrane protein with a region containing five hydrophobic segments within the C-terminal part of the protein, suggesting that M83 is a five-span transmembrane molecule. The M83 protein was expressed on the cell surface as a glycosylated protein with a molecular mass of 84 kDa. The M83 gene was localized to human chromosome 16p13.3, mouse chromosome 17B1, and rat chromosome 10q12.3 distal. In human, M83 mRNA was highly expressed in placenta, pancreas, and lymphohematopoietic tissues including peripheral blood, spleen, and bone marrow. Among hematopoietic cells, it was highly expressed in resting T lymphocytes and was downregulated by cell activation, suggestive of its biological role related to the T cell resting status.
Keywords: five-span transmembrane protein; chromosomal mapping; resting T lymphocyte
Rights: © 2000 Academic Press
RMID: 0001000529
DOI: 10.1006/bbrc.2000.3409
Appears in Collections:Paediatrics publications

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