Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/7008
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dc.contributor.authorHill, W.-
dc.contributor.authorHarper, G.-
dc.contributor.authorRozaklis, T.-
dc.contributor.authorHopwood, J.-
dc.date.issued1997-
dc.identifier.citationBiochemical and molecular medicine, 1997; 62(1):85-94-
dc.identifier.issn1077-3150-
dc.identifier.issn1095-5577-
dc.identifier.urihttp://hdl.handle.net/2440/7008-
dc.description.abstractCystic fibrosis is associated with mutations of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-regulated plasma membrane chloride channel. Cystic fibrosis patients have been reported to possess elevated sulfation of glycoconjugates, which may contribute to the pathogenesis of the disease. Sulfation of glycosaminoglycans by a cystic fibrosis pancreatic adenocarcinoma cell line homozygous for DeltaF508 (CFPAC-1), a control pancreatic cell line (PANC-1), two CFPAC-1 cell lines transfected with the gene for CFTR (PLJ-CFTR-4.7, TR20), and a mock-transfected CFPAC-1 control (PLJ-6) was investigated. Cells were radiolabeled with [35S]sulfate and [3H]glucosamine, and glycosaminoglycans secreted into the medium after 24 and 72 h were isolated. Chondroitinase ABC digestion of chondroitin/dermatan sulfate allowed the recovery of disaccharides which were analyzed for their degree of sulfation by strong anion-exchange HPLC. No differences in the extent of sulfation by any of the cell lines were noted. However, glycoaminoglycans synthesized by cystic fibrosis cells consistently exhibited twofold higher [35S]-sulfate:[3H]glucosamine ratios than the controls. We conclude that CFTR plays no role in the sulfation of chondroitin/dermatan sulfate by pancreatic cells and that isotope incorporation ratios alone are insufficient evidence of changes in sulfation levels.-
dc.description.statementofresponsibilityWarren G. Hill, Gregory S. Harper, Tina Rozaklis, John J. Hopwood-
dc.language.isoen-
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCE-
dc.rightsCopyright © 1997, Elsevier-
dc.subjectcystic fibrosis-
dc.subjectCFTR-
dc.subjectsulfation-
dc.subjectglycosaminoglycans-
dc.subjectpancreatic cells-
dc.titleSulfation of chondroitin/dermatan sulfate by cystic fibrosis pancreatic duct cells is not different from control cells-
dc.typeJournal article-
dc.identifier.doi10.1006/bmme.1997.2625-
pubs.publication-statusPublished-
Appears in Collections:Aurora harvest
Paediatrics publications

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