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Type: Journal article
Title: QCM-D fingerprinting of membrane-active peptides
Author: McCubbin, G.
Praporski, S.
Piantavigna, S.
Knappe, D.
Hoffmann, R.
Bowie, J.
Separovic, F.
Martin, L.
Citation: European Biophysics Journal With Biophysics Letters, 2011; 40(4):437-446
Publisher: Springer-Verlag
Issue Date: 2011
ISSN: 0175-7571
Statement of
George A. McCubbin, Slavica Praporski, Stefania Piantavigna, Daniel Knappe, Ralf Hoffmann, John H. Bowie, Frances Separovic, Lisandra L. Martin
Abstract: The increasing prevalence of antibiotic-resistant bacteria is becoming a public health crisis. Antimicrobial peptides (AMPs) are a promising solution, because bacterial resistance is less likely. Quartz crystal microbalance with dissipation monitoring (QCM-D) is a versatile and valuable technique for investigation of these peptides. This article looks at the different approaches to the interpretation of QCM-D data, showing how to extract the maximum information from the data. Five AMPs of diverse charge, length and activity are used as case studies: caerin 1.1 wild-type, two caerin 1.1 mutants (Gly15Gly19-caerin 1.1 and Ala15Ala19-caerin 1.1), aurein 1.2 and oncocin. The interaction between the AMP and a 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membrane is analysed inter alia using frequency-dissipation plots (∆f-∆D plots) to ascertain the mechanism of action of the AMP. The ∆f-∆D plot can then be used to provide a fingerprint for the AMP-membrane interaction. Building up a database of these fingerprints for all known AMPs will enable the relationship between AMP structure and membrane activity to be better understood, hopefully leading to the future development of antibiotics without bacterial resistance.
Keywords: Antimicrobial peptide; Quartz crystal microbalance; Frequency–dissipation plot; Membrane pore; Membrane disruption
Rights: © European Biophysical Societies’ Association 2010
RMID: 0020105752
DOI: 10.1007/s00249-010-0652-5
Appears in Collections:Chemistry publications

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