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Type: Journal article
Title: Immune response to enzyme replacement therapy: clinical signs of hypersensitivity reactions and altered enzyme distribution in a high titre rat model
Author: Brooks, D.
Hopwood, J.
King, B.
Citation: Biochimica et Biophysica Acta, 1998; 1407(2):163-172
Issue Date: 1998
ISSN: 0304-4165
Abstract: Immune responses to enzyme replacement therapy (ERT) have been reported and can result in a hypersensitivity/anaphylactic reaction during or immediately after enzyme infusion. We have investigated the infusion of the lysosomal enzyme N-acetylgalactosamine 4-sulphatase (4-sulphatase) into immunized, high titre rats as a model of immune response to ERT. To simulate ERT, high and low titre rats were infused with different doses of radiolabelled recombinant human 4-sulphatase (3H-rh4S). There was evidence of altered targeting, inactivation and degradation of 4-sulphatase in high titre (titre 1024000) compared to low titre (titre 64) rats. There was more 4-sulphatase enzyme activity detected in 5 mg/kg high titre rats when compared to 1 mg/kg high titre rats, suggesting that the antibodies could be saturable in vivo. However, the rats treated with 5 mg/kg 3H-rh4S all had clinical signs of hypersensitivity reactions to 4-sulphatase infusion. There were no apparent signs of adverse reactions in either the high titre 1 mg/kg rats or the low titre rats (1, 5 mg/kg). The high titre 5 mg/kg rats also had changes in 3H-rh4S distribution, with lower levels delivered to the liver and a marked increase in the level remaining in plasma, when compared to either 1 mg/kg high titre rats or low titre rats (1, 5 mg/kg).
Keywords: Liver; Animals; Rats; Metabolism, Inborn Errors; Mucopolysaccharidosis VI; Hypersensitivity; Anaphylaxis; Disease Models, Animal; Sulfatases; N-Acetylgalactosamine-4-Sulfatase; Recombinant Proteins; Centrifugation, Density Gradient; Immunity
RMID: 0030005730
DOI: 10.1016/S0925-4439(98)00034-9
Appears in Collections:Paediatrics publications

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