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Type: Journal article
Title: A membrane protein primarily associated with the lysosomal compartment
Author: Brooks, D.
Bradford, T.
Carlsson, S.
Hopwood, J.
Citation: Biochimica et Biophysica Acta, 1997; 1327(2):162-170
Issue Date: 1997
ISSN: 0304-4165
Abstract: A monoclonal antibody designated MBR 39 has been generated against a membrane associated protein found selectively on lysosomes. MBR 39 reacts with the cytosolic face of the lysosome and was used to develop an organelle binding assay which reacted with high density organelles characteristic of lysosomes. These organelles contained lysosomal enzyme markers which included the integral membrane protein acetyl-CoA:alpha-glucosaminide N-acetyltransferase and the soluble lysosomal enzyme markers acid phosphatase (mature form), beta-hexosaminidase, arylsulfatase, and alpha-L-iduronidase. Under conditions which disrupt lysosomes the release of the latter soluble lysosomal enzymes was demonstrated from MBR 39 bound organelles. Immunoblots of MBR 39 with purified fibroblast lysosomal membrane, demonstrated reactivity with polypeptides of molecular mass 63 kDa (major species) and 73 kDa (minor species).
Keywords: Lysosomes; Cytosol; Humans; Membrane Proteins; Antibodies, Monoclonal; Cell Compartmentation
RMID: 0030005727
DOI: 10.1016/S0005-2736(97)00053-9
Appears in Collections:Paediatrics publications

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