Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7033
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dc.contributor.authorBrooks, D.en
dc.contributor.authorBradford, T.en
dc.contributor.authorCarlsson, S.en
dc.contributor.authorHopwood, J.en
dc.date.issued1997en
dc.identifier.citationBiochimica et Biophysica Acta, 1997; 1327(2):162-170en
dc.identifier.issn0304-4165en
dc.identifier.issn1878-2434en
dc.identifier.urihttp://hdl.handle.net/2440/7033-
dc.description.abstractA monoclonal antibody designated MBR 39 has been generated against a membrane associated protein found selectively on lysosomes. MBR 39 reacts with the cytosolic face of the lysosome and was used to develop an organelle binding assay which reacted with high density organelles characteristic of lysosomes. These organelles contained lysosomal enzyme markers which included the integral membrane protein acetyl-CoA:alpha-glucosaminide N-acetyltransferase and the soluble lysosomal enzyme markers acid phosphatase (mature form), beta-hexosaminidase, arylsulfatase, and alpha-L-iduronidase. Under conditions which disrupt lysosomes the release of the latter soluble lysosomal enzymes was demonstrated from MBR 39 bound organelles. Immunoblots of MBR 39 with purified fibroblast lysosomal membrane, demonstrated reactivity with polypeptides of molecular mass 63 kDa (major species) and 73 kDa (minor species).en
dc.language.isoenen
dc.publisherELSEVIER SCIENCE BVen
dc.subjectLysosomes; Cytosol; Humans; Membrane Proteins; Antibodies, Monoclonal; Cell Compartmentationen
dc.titleA membrane protein primarily associated with the lysosomal compartmenten
dc.typeJournal articleen
dc.identifier.rmid0030005727en
dc.identifier.doi10.1016/S0005-2736(97)00053-9en
dc.identifier.pubid69733-
pubs.library.collectionPaediatrics publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidBrooks, D. [0000-0001-9098-3626]en
Appears in Collections:Paediatrics publications

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