Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/70400
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Type: Journal article
Title: Comparison of methods for the analysis of smoke related phenols and their conjugates in grapes and wine
Author: Wilkinson, K.
Ristic, R.
Pinchbeck, K.
Fudge, A.
Singh, D.
Pitt, K.
Downey, M.
Baldock, G.
Hayasaka, Y.
Parker, M.
Herderich, M.
Citation: Australian Journal of Grape and Wine Research, 2011; 17(2):S22-S28
Publisher: Australian Society of Viticulture and Oenology
Issue Date: 2011
ISSN: 1322-7130
1755-0238
Statement of
Responsibility: 
K. L. Wilkinson, R. Ristic, K. A. Pinchbeck, A. L. Fudge, D. P. Singh, K. M. Pitt, M. O. Downey, G. A. Baldock, Y. Hayasaka, M. Parker and M. J. Herderich
Abstract: BACKGROUND AND AIMS: Australian grape growers and winemakers have typically relied on guaiacol and 4-methylguaiacol measurements to determine smoke exposure of grapes following bushfires or prescribed burns. However, the guaiacol and 4-methylguaiacol content of grapes does not always correlate with the extent of taint in resultant wines. This study compared several methods for the analysis of smoke related phenols and their conjugates in grapes and wine, to determine their capacity as diagnostic assays for smoke exposure. METHODS AND RESULTS: Smoke-affected grapes were sourced from commercial vineyards exposed to bushfire smoke and from experimental field trials involving the application of smoke to grapevines, and small-scale wines were made from a number of these samples. Several analytical methods were applied to grapes and wine to determine the concentration of smoke related phenols and their conjugates. Strong correlations were observed between the glycoconjugate content of smoke-affected grapes and the concentration of guaiacol and 4-methylguaiacol released following acid hydrolysis of juice. CONCLUSIONS: Where smoke-affected grapes contain low or non-detectable levels of guaiacol and 4-methylguaiacol, analytical methods that quantify their glycoconjugate forms (either directly or indirectly) provide a better indication of the extent of smoke exposure. SIGNIFICANCE OF THE STUDY: This is the first study to compare different methods for assessing smoke exposure in grapes and wine, through analysis of free and bound guaiacol and 4-methylguaiacol. These methods will allow grape growers and winemakers to more reliably assess smoke exposure of grapes, enabling better informed decisions to be made with regards to harvesting and processing smoke-affected grapes.
Rights: © 2011 Australian Society of Viticulture and Oenology Inc
RMID: 0020109435
DOI: 10.1111/j.1755-0238.2011.00147.x
Grant ID: http://purl.org/au-research/grants/arc/LP0989138
Appears in Collections:Agriculture, Food and Wine publications

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