Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7041
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Type: Journal article
Title: Cord blood neutrophil responses to polyunsaturated fatty acids: effects on degranulation and oxidative respiratory burst
Author: Ferrante, A.
Carman, K.
Nandoskar, M.
McPhee, A.
Poulos, A.
Citation: Biology of the Neonate, 1996; 69(6):368-375
Publisher: KARGER
Issue Date: 1996
ISSN: 0006-3126
1661-7819
Abstract: Lipid mediators such as arachidonic acid (AA) generated during inflammation play an important role in stimulating phagocytic cell responses. Since cord blood neutrophils show reduced responses to agonists such as the bacterial tripeptide f-Met-Leu-Phe (fMLP), it would be of interest to know whether cord blood neutrophils show normal or reduced responses to AA and other fatty acids. The data showed that the polyunsaturated n-6 fatty acid (PUFA) AA stimulated cord blood neutrophils to produce a respiratory response (measured by chemiluminescence) and degranulation. Other PUFAs, eicosapentanoic acid and docosahexaenoic acid, elicited similar responses in cord blood neutrophils. Specific granule release and chemiluminescence response in cord blood neutrophils were evident at 0.1-0.5 microgram/ml of PUFA, concentrations normally found in vivo during inflammation or following diets enriched with n-3 fatty acids. Neutrophil responses to PUFA were significantly better than those to either fMLP or phorbol myristate acetate. Cord blood neutrophils primed with PUFA showed enhanced responses to fMLP. These results suggest that cord blood neutrophils respond to a similar degree to adult neutrophils to the AA which is generated during the inflammatory response and to the n-3 fatty acids eicosapentaenoic acid/docosahexaenoic acid, both of which may be used in diet manipulation of neurological function and immunological reactions.
Keywords: Neutrophils; Cytoplasmic Granules; Fetal Blood; Humans; Tetradecanoylphorbol Acetate; Fatty Acids, Omega-3; Docosahexaenoic Acids; Eicosapentaenoic Acid; Fatty Acids, Unsaturated; Arachidonic Acid; N-Formylmethionine Leucyl-Phenylalanine; Luminescent Measurements; Respiratory Burst; Adult; Infant, Newborn
RMID: 0030005725
DOI: 10.1159/000244333
Appears in Collections:Paediatrics publications

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