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|Title:||Identification of self-incompatibility genotypes and DNA fingerprinting of some Australian almond cultivars|
|Citation:||Proceedings of the Vth International Symposium on Pistachios and Almonds : Sanliurfa, Turkey, October 13-17, 2009 / B. E. Ak, M. Wirthensohn and T. Gradziel (eds.): pp.641-643|
|Series/Report no.:||ISHS Acta Horticulturae: 912|
|Conference Name:||International Symposium on Pistachios and Almonds (5th : 2009 : Sanliurfa, Turkey)|
|M. Wirthensohn, M. Rahemi and A. Fernández i Martí|
|Abstract:||The origin of Australian almond cultivars is mostly unknown and therefore the aim of this study is to characterise them for their incompatibility groups and their genetic diversity. The self incompatibility (SI) genotypes of 25 Australian almond (Prunus dulcis) cultivars were determined by PCR analysis of genomic DNA using a combination of specific primers based on the intron regions and primers based on the conserved regions of Rosaceous S-RNase genes. DNA fingerprinting of the cultivars was achieved through microsatellite fragment analysis and comparison with European and American cultivars, to determine the genetic diversity within Australian almond accessions. Results showed a diverse range of incompatibility groups within Australian cultivars and fingerprinting reflected their ancestry, a combination of American and European backgrounds. A germplasm collection has been established for conservation and further characterisation of local cultivars.|
|Keywords:||Prunus dulcis; SSR marker; microsatellite; polymorphism; S-allele; incompatibility|
|Description:||The original publication is available at www.actahort.org|
|Appears in Collections:||Agriculture, Food and Wine publications|
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