Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/70528
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, W. | - |
dc.contributor.author | Kohler, B. | - |
dc.contributor.author | Cao, F. | - |
dc.contributor.author | Liu, G. | - |
dc.contributor.author | Gong, Y. | - |
dc.contributor.author | Sheng, S. | - |
dc.contributor.author | Song, Q. | - |
dc.contributor.author | Cheng, X. | - |
dc.contributor.author | Garnett, T. | - |
dc.contributor.author | Okamoto, M. | - |
dc.contributor.author | Qin, R. | - |
dc.contributor.author | Mueller-Roeber, B. | - |
dc.contributor.author | Tester, M. | - |
dc.contributor.author | Liu, L. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | New Phytologist, 2012; 193(2):432-444 | - |
dc.identifier.issn | 0028-646X | - |
dc.identifier.issn | 1469-8137 | - |
dc.identifier.uri | http://hdl.handle.net/2440/70528 | - |
dc.description.abstract | • Despite the great agricultural and ecological importance of efficient use of urea-containing nitrogen fertilizers by crops, molecular and physiological identities of urea transport in higher plants have been investigated only in Arabidopsis. • We performed short-time urea-influx assays which have identified a low-affinity and high-affinity (K(m) of 7.55 μM) transport system for urea-uptake by rice roots (Oryza sativa). • A high-affinity urea transporter OsDUR3 from rice was functionally characterized here for the first time among crops. OsDUR3 encodes an integral membrane-protein with 721 amino acid residues and 15 predicted transmembrane domains. Heterologous expression demonstrated that OsDUR3 restored yeast dur3-mutant growth on urea and facilitated urea import with a K(m) of c. 10 μM in Xenopus oocytes. • Quantitative reverse-transcription polymerase chain reaction (qPCR) analysis revealed upregulation of OsDUR3 in rice roots under nitrogen-deficiency and urea-resupply after nitrogen-starvation. Importantly, overexpression of OsDUR3 complemented the Arabidopsis atdur3-1 mutant, improving growth on low urea and increasing root urea-uptake markedly. Together with its plasma membrane localization detected by green fluorescent protein (GFP)-tagging and with findings that disruption of OsDUR3 by T-DNA reduces rice growth on urea and urea uptake, we suggest that OsDUR3 is an active urea transporter that plays a significant role in effective urea acquisition and utilisation in rice. | - |
dc.description.statementofresponsibility | Wei-Hong Wang, Barbara Köhler, Feng-Qiu Cao, Guo-Wei Liu, Yuan-Yong Gong, Song Sheng, Qi-Chao Song, Xiao-Yuan Cheng, Trevor Garnett, Mamoru Okamoto, Rui Qin, Bernd Mueller-Roeber, Mark Tester, and Lai-Hua Liu | - |
dc.language.iso | en | - |
dc.publisher | Blackwell Publishing Ltd | - |
dc.rights | © 2011 The Authors. New Phytologist © 2011 New Phytologist Trust | - |
dc.source.uri | http://dx.doi.org/10.1111/j.1469-8137.2011.03929.x | - |
dc.subject | high-affinity transporter | - |
dc.subject | leaf senescence | - |
dc.subject | nitrogen remobilization | - |
dc.subject | OsDUR3 | - |
dc.subject | overexpression | - |
dc.subject | rice plant | - |
dc.subject | urea transport and utilization. | - |
dc.title | Rice DUR3 mediates high-affinity urea transport and plays an effective role in improvement of urea acquisition and utilization when expressed in Arabidopsis | - |
dc.type | Journal article | - |
dc.contributor.organisation | Australian Centre for Plant Functional Genomics (ACPFG) | - |
dc.identifier.doi | 10.1111/j.1469-8137.2011.03929.x | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Garnett, T. [0000-0003-1664-9659] | - |
dc.identifier.orcid | Okamoto, M. [0000-0002-2989-607X] | - |
Appears in Collections: | Aurora harvest Australian Centre for Plant Functional Genomics publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.