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Type: Journal article
Title: Production and characterization of monoclonal antibody to bovine leukemia virus gp51-SU
Author: Jafari Jozani, R.
Khazraiinia, P.
Taheri, M.
Hemmatzadeh, F.
Ashrafihelan, J.
Citation: Global Veterinaria, 2011; 7(2):158-163
Publisher: IDOSI
Issue Date: 2011
ISSN: 1992-6197
Statement of
Raziallah Jafari Joozani, Parvaneh Khazrai Nia, Mohammad Taheri, Farhid Hemmatzadeh and Javad Ashrafihelan
Abstract: Enzootic bovine leukemia (EBL) is a disease of cattle with worldwide distribution. The bovine leukemia virus (BLV), is a typical type C retrovirus, has four genes: gag, pol, pro and env, flanked by long terminal repeats (LTR) and lacking oncogenes. Gene env encodes a precursor protein named Pr72 env which undergoes glycosylation and lysis, giving rise to an envelope glycoprotein gp51-SU with a total of 12 epitopes. The objective of the present study was to produce a panel of monoclonal antibodies (mAbs) against BLV-gp51-SU following the standard immunization protocols. The antigen preparations included cellular lysates, a fraction of cellular lysates to study antibodies against BLV proteins precursors, whole virus particles and semipurified gp51 -SU. Both route of injection (SC and IP) resulted in hyperimmunized mice. As indicated by indirect-ELISA and also yielded high fusion efficiency. The hybridoma colonies were stable and had a tendency to save their ability to secrete antibodies. Fusions produced five positive colonies in screening tests, two of which were against gp51-SU. The targeted hydrophobic domain of the gp51-SU molecule was detected by one of the colonies of mAbs. The two interested mAbs were used for immunohistochemistry on lymph node sections of a cow with lymphosarcoma which can detect immunoreactive materials in sections and also in H&E staining. We conclude the produced panel of mAbs is able to be used in detection of gp51-SU and a new epitope identified on this molecule could be used in IHC method for diagnosis of BLV infection. © IDOSI Publications, 2011.
Keywords: Bovine Leukemia Virus
Monoclonal antibody
Rights: © IDOSI Publications, 2011
Appears in Collections:Animal and Veterinary Sciences publications
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