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Type: Journal article
Title: Defining regions of loss of heterozygosity of 16q in breast cancer cell lines
Author: Callen, D.
Crawford, J.
Derwas, C.
Cleton-Jansen, A.
Cornelisse, C.
Baker, E.
Citation: Cancer Genetics, 2002; 133(1):76-82
Publisher: Elsevier Science Inc
Issue Date: 2002
ISSN: 0165-4608
Statement of
David F. Callen, Joanne Crawford, Cathy Derwas, Anne-Marie Cleton-Jansen, Cees J. Cornelisse, Elizabeth Baker
Abstract: The loss of heterozygosity (LOH) of chromosome 16 was assessed in 21 breast cancer cell lines and two nontumorigenic breast epithelial cell lines by typing microsatellite markers distributed on this chromosome. In addition, dual-color fluorescence in situ hybridization was used to metaphase spreads of these cell lines using chromosome 16 paint and region specific probes. Eleven of the cell lines had LOH for chromosome 16, two for the entire chromosome, three for the long arm, and six had LOH for restricted regions of the long arm. The results supported evidence that there are two predominant regions of LOH, 16q22.1 and 16q24.3. The cell lines with chromosome 16 LOH can be used for screening candidate tumor suppressor genes at 16q in breast cancer.
Keywords: Tumor Cells, Cultured
Chromosomes, Human, Pair 16
Breast Neoplasms
In Situ Hybridization, Fluorescence
Loss of Heterozygosity
Description: © 2002 Elsevier Science Inc. All rights reserved.
DOI: 10.1016/S0165-4608(01)00565-9
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