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Type: Journal article
Title: Dot-blot enzyme immunoassay for the detection of bovine viral diarrhea virus antibodies
Author: Hemmatzadeh, F.
Amini, F.
Citation: Veterinarski Arhiv, 2009; 79(4):343-350
Publisher: Univ Zagreb Vet Faculty
Issue Date: 2009
ISSN: 0372-5480
Statement of
Farhid Hemmatzadeh and Farhad Amini
Abstract: Dot blot enzyme immunoassay (DB-EIA) was utilized for the detection of bovine viral diarrhea (BVD) antibodies in infected cattle. In this assay whole particles of the NADL strain of BVD virus were used as an antigen. A total of 1250 sera from SN and ELISA tested cows were tested for BVDV antibodies. HRPOconjugated protein G was used to detect bound BVDV antibodies using TMB as the substrate. Statistical analyses did not show any signififi cant differences in the sensitivity of DB-EIA, to the ELISA and SN tests. The assy proved to be a simple, inexpensive, reliable and rapid tool for BVD serodiagnosis.
Keywords: Dot-blot enzyme immunoassays; bovine viral diarrhea; serum neutralization test; ELISA
Rights: Copyright status unknown
RMID: 0020108330
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Appears in Collections:Animal and Veterinary Sciences publications

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