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Type: Journal article
Title: Purification, crystallization and preliminary X-ray crystallographic analysis of the putative Vibrio parahaemolyticus resuscitation-promoting factor YeaZ
Author: Aydin, I.
Dimitropoulos, A.
Chen, S.
Thomas, C.
Roujeinikova, A.
Citation: Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2011; 67(5):604-607
Publisher: Blackwell Munksgaard
Issue Date: 2011
ISSN: 1744-3091
Statement of
I. Aydin, A. Dimitropoulos, S.H. Chen, C. Thomas and A. Roujeinikova
Abstract: Vibrio parahaemolyticus is a human pathogen associated with gastroenteritis caused by the ingestion of contaminated raw seafood. V. parahaemolyticus is able to survive exposure to low temperatures typical of those used for the refrigeration of foods by entering a viable but nonculturable (VBNC) state. The VBNC cells can regain culturability and renewed ability to cause infection upon temperature upshift. The resuscitation-promoting factor protein (Rpf, YeaZ) plays a key role in reactivation of growth. Crystals of V. parahaemolyticus YeaZ have been grown using the hanging-drop vapour-diffusion method with polyethylene glycol as a precipitating agent. The crystals belonged to the primitive monoclinic space group P21, with unit-cell parameters a = 81.7, b = 63.8, c = 82.3 Å, = 105° and four subunits in the asymmetric unit. A complete X-ray diffraction data set was collected from a single crystal to 3.1 Å resolution.
Keywords: YeaZ
resuscitation-promoting factors
Vibrio parahaemolyticus
Rights: Copyright © International Union of Crystallography
DOI: 10.1107/S1744309111010219
Grant ID: ARC
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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