Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/71147
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Type: Journal article
Title: Gamma-irradiated influenza virus uniquely induces IFN-I mediated lymphocyte activation independent of the TLR7/MyD88 pathway
Author: Furuya, Y.
Chan, J.
Wan, E.
Koskinen, A.
Diener, K.
Hayball, J.
Regner, M.
Mullbacher, A.
Alsharifi, M.
Citation: PLoS One, 2011; 6(10):e25765-1-e25765-12
Publisher: Public Library of Science
Issue Date: 2011
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Yoichi Furuya, Jennifer Chan, En-Chi Wan, Aulikki Koskinen, Kerrilyn R. Diener, John D. Hayball, Matthias Regner, Arno Müllbacher, Mohammed Alsharifi
Abstract: Background: We have shown previously in mice, that infection with live viruses, including influenza/A and Semliki Forest virus (SFV), induces systemic partial activation of lymphocytes, characterized by cell surface expression of CD69 and CD86, but not CD25. This partial lymphocytes activation is mediated by type-I interferons (IFN-I). Importantly, we have shown that c-irradiated SFV does not induce IFN-I and the associated lymphocyte activation. Principal Findings: Here we report that, in contrast to SFV, c-irradiated influenza A virus elicits partial lymphocyte activation in vivo. Furthermore, we show that when using influenza viruses inactivated by a variety of methods (UV, ionising radiation and formalin treatment), as well as commercially available influenza vaccines, only c-irradiated influenza virus is able to trigger IFN-I-dependent partial lymphocyte activation in the absence of the TLR7/MyD88 signalling pathways. Conclusions: Our data suggest an important mechanism for the recognition of c-irradiated influenza vaccine by cytosolic receptors, which correspond with the ability of c-irradiated influenza virus to induce cross-reactive and cross-protective cytotoxic T cell responses.
Keywords: Lymphocytes; T-Lymphocytes, Cytotoxic; Cell Line; Animals; Dogs; Mice; Neuraminidase; Membrane Glycoproteins; Interferon Type I; Hemagglutinin Glycoproteins, Influenza Virus; Virus Activation; Signal Transduction; Gamma Rays; Female; Cricetinae; Toll-Like Receptor 7; Influenza A Virus, H3N2 Subtype; Influenza A Virus, H1N1 Subtype; Myeloid Differentiation Factor 88
Rights: © 2011 Furuya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020113525
DOI: 10.1371/journal.pone.0025765
Appears in Collections:Molecular and Biomedical Science publications

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