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https://hdl.handle.net/2440/7118
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Type: | Journal article |
Title: | Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry |
Author: | Kumaratilake, L. Ferrante, A. |
Citation: | Clinical and Vaccine Immunology, 2000; 7(1):9-13 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2000 |
ISSN: | 1071-412X 1098-6588 |
Abstract: | A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software. To differentiate the proportion of neutrophil surface-bound merozoites from the merozoites ingested by neutrophils, the fluorescence of bound merozoites was quenched by adding trypan blue. The data showed that sera from malaria-immune individuals in the Solomon Islands and Papua New Guinea promoted merozoite engulfment by neutrophils. The cytokines tumor necrosis factor alpha, gamma interferon, granulocyte-macrophage colony-stimulating factor, and interleukin-1beta significantly increased the amount and the rate of merozoite phagocytosis by neutrophils. Optimum merozoite phagocytosis occurred when both cytokines and anti-malarial antibody were present. |
Keywords: | Neutrophils Phagocytes Animals Humans Plasmodium falciparum Trypan Blue Fluorescein-5-isothiocyanate Merozoite Surface Protein 1 Recombinant Proteins Antibodies Cytokines Flow Cytometry Phagocytosis Fluorescence Time Factors Software Melanesia Papua New Guinea Opsonin Proteins Interferon-gamma |
DOI: | 10.1128/cdli.7.1.9-13.2000 |
Published version: | http://dx.doi.org/10.1128/cdli.7.1.9-13.2000 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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