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Type: Journal article
Title: Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry
Author: Kumaratilake, L.
Ferrante, A.
Citation: Clinical and Diagnostic Laboratory Immunology, 2000; 7(1):9-13
Publisher: Amer Soc Microbiology
Issue Date: 2000
ISSN: 1071-412X
Abstract: A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software. To differentiate the proportion of neutrophil surface-bound merozoites from the merozoites ingested by neutrophils, the fluorescence of bound merozoites was quenched by adding trypan blue. The data showed that sera from malaria-immune individuals in the Solomon Islands and Papua New Guinea promoted merozoite engulfment by neutrophils. The cytokines tumor necrosis factor alpha, gamma interferon, granulocyte-macrophage colony-stimulating factor, and interleukin-1beta significantly increased the amount and the rate of merozoite phagocytosis by neutrophils. Optimum merozoite phagocytosis occurred when both cytokines and anti-malarial antibody were present.
Keywords: Neutrophils; Phagocytes; Animals; Humans; Plasmodium falciparum; Trypan Blue; Fluorescein-5-isothiocyanate; Merozoite Surface Protein 1; Recombinant Proteins; Antibodies; Cytokines; Flow Cytometry; Phagocytosis; Fluorescence; Time Factors; Software; Melanesia; Papua New Guinea; Opsonin Proteins; Interferon-gamma
RMID: 0001000246
DOI: 10.1128/cdli.7.1.9-13.2000
Appears in Collections:Paediatrics publications

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