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Type: Journal article
Title: Rapid and simultaneous quantification of rhamnose, mannitol, and lactulose in urine by HPLC for estimating intestinal permeability in pediatric practice
Author: Miki, K.
Butler, R.
Moore, D.
Davidson, G.
Citation: Clinical Chemistry (Washington, DC): international journal of molecular diagnostics and laboratory medicine, 1996; 42(1):71-75
Publisher: Oxford University Press (OUP)
Issue Date: 1996
ISSN: 0009-9147
Statement of
Miki, K; Butler, R; Moore, D; Davidson, G
Abstract: Determination of intestinal permeability by measuring nonmetabolized sugars has been used to assess the integrity of intestinal mucosa. We have developed and validated a modified HPLC method for determining the concentration of L-rhamnose, mannitol, and lactulose in urine, using an amine-modified silica column and refractive index detection. Probe sugars are simultaneously resolved within 18 min. The calibration curve for each sugar is linear to 20 mmol/L. The minimum detectable concentration of lactulose is 0.05 mmol/L. Recovery of probe sugars is between 99.3% and 105.1%. Overall precision (CV) of estimation of probe sugars ranges from 4.2% to 6.5%. In 14 urine samples from healthy children who ingested the test solution containing 1 g of L-rhamnose, 1 g of mannitol, and 5 g of lactulose, the 5-h urinary excretion ratios of lactulose/rhamnose and lactulose/mannitol were 0.047 +/- 0.018 and 0.021 +/- 0.010 (mean +/- SD), respectively. This method presents a rapid, convenient, and practical technique for determining intestinal permeability in clinical pediatric practice.
Keywords: Intestines
Chromatography, High Pressure Liquid
Reference Values
Rights: © 1996 The American Association for Clinical Chemistry
DOI: 10.1093/clinchem/42.1.71
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Appears in Collections:Aurora harvest 5
Paediatrics publications

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