Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Methods for the purification and characterization of human adipose-derived stem cells.|
|Citation:||Methods in Molecular Biology: Adipose-Derived Stem Cells - Methods and Protocols, 2011 / Gimble, J., Bunnell, B. (ed./s), pp.109-120|
|Stan Gronthos and Andrew C.W. Zannettino|
|Abstract:||Peripheral adipose tissue contains a population of clonogenic precursor cells referred to as adipose-derived stem cells (ASC) that retain the capacity to differentiate into multiple cell types including osteoblasts, adipocytes, chondrocytes, myocytes, and neuronal cells following ex vivo expansion. Recent studies have demonstrated that ASC are most likely derived from a perivascular niche within highly vascularised fat tissue, analogous to different mesenchymal cell populations identified in other tissues throughout the body. The following chapter describes techniques to prospectively isolate clonogenic ASC from adult human adipose tissue using antibodies directed against perivascular markers and methods to immunophenotypically characterize their ex vivo expanded progeny.|
|Keywords:||Bone marrow stromal stem cells; mesenchymal stem cells; magnetic-activated cell sorting; fluorescence-activated cell sorting|
|Description:||Methods in Molecular Biology; no. 702|
|Appears in Collections:||Medicine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.