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Type: Book chapter
Title: Methods for the purification and characterization of human adipose-derived stem cells.
Author: Gronthos, S.
Zannettino, A.
Citation: Methods in Molecular Biology: Adipose-Derived Stem Cells - Methods and Protocols, 2011 / Gimble, J., Bunnell, B. (ed./s), pp.109-120
Publisher: Humana Press
Publisher Place: USA
Issue Date: 2011
ISBN: 9781617379598
Statement of
Stan Gronthos and Andrew C.W. Zannettino
Abstract: Peripheral adipose tissue contains a population of clonogenic precursor cells referred to as adipose-derived stem cells (ASC) that retain the capacity to differentiate into multiple cell types including osteoblasts, adipocytes, chondrocytes, myocytes, and neuronal cells following ex vivo expansion. Recent studies have demonstrated that ASC are most likely derived from a perivascular niche within highly vascularised fat tissue, analogous to different mesenchymal cell populations identified in other tissues throughout the body. The following chapter describes techniques to prospectively isolate clonogenic ASC from adult human adipose tissue using antibodies directed against perivascular markers and methods to immunophenotypically characterize their ex vivo expanded progeny.
Keywords: Bone marrow stromal stem cells; mesenchymal stem cells; magnetic-activated cell sorting; fluorescence-activated cell sorting
Description: Methods in Molecular Biology; no. 702
RMID: 0020110899
DOI: 10.1007/978-1-61737-960-4_9
Appears in Collections:Medicine publications

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