Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7199
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Type: Journal article
Title: Cytokine receptor expression in human lymphoid tissue: analysis by fluorescence microscopy
Author: Zola, H.
Ridings, J.
Weedon, H.
Fusco, M.
Byard, R.
Macardle, P.
Citation: Disease Markers, 1994; 12(2):147-165
Publisher: Hindawi Publishing Corporation
Issue Date: 1994
ISSN: 0278-0240
1875-8630
Statement of
Responsibility: 
Heddy Zola, Jodie Ridings, Helen Weedon, Michael Fusco, Roger W. Byard, Peter J. Macardle
Abstract: A highly-sensitive flourescence method, capable of detecting cytokine receptors present at low concentrations (around I DO molecules per cell) by flow cytometry, was adapted for use on tissue sections. This method was used to examine the expression of several cytokine receptors in lymphoid ti ss ues. lL-2 receptors were distributed broadly, with higher concentrations in T cell areas. lL-1 receptor Type I was detected in T cell areas and in the follicular mantle, and was strongly expressed on vasc ular endothelium. IL-6 receptor was found at very low concentration, both within and outside germinal centres. The gp 130 molecule, which is involved in the functional receptor complex for IL-6 and several other cytokines, was present at higher concentrations, particularly in the germinal centre. Analysis of receptor expression in secondary lymphoid tissue provides evidence bearing on the physiological roles of cytokines, as these tissues contain cells at various stages of physiological activation located in well-defined functional zones.
Keywords: Cytokine receptors; Interleukin receptors; Tissue staining; Cy3 fluorescence
Rights: Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030005665
DOI: 10.1155/1994/563543
Appears in Collections:Paediatrics publications

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