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|Title:||Label-free reflectometric interference microchip biosensor based on nanoporous alumina for detection of circulating tumour cells|
|Citation:||Biosensors & Bioelectronics, 2012; 35(1):167-173|
|Publisher:||Elsevier Advanced Technology|
|Tushar Kumeria, Mahaveer D. Kurkuri, Kerrilyn R. Diener, Luke Parkinson and Dusan Losic|
|Abstract:||In this report, a label-free reflectometric interference spectroscopy (RIfS) based microchip biosensor for the detection of circulating tumour cells (CTCs) is demonstrated. Highly ordered nanoporous anodic aluminium oxide (AAO) fabricated by electrochemical anodization of aluminium foil was used as the RIfS sensing platform. Biotinylated anti-EpCAM antibody that specifically binds to human cancer cells of epithelial origin such as pancreatic cancer cells (PANC-1) was covalently attached to the AAO surface through multiple surface functionalization steps. Whole blood or phosphate buffer saline spiked with low numbers of pancreatic cancer cells were successfully detected by specially designed microfluidic device incorporating an AAO RIfS sensor, without labour intensive fluorescence labelling and/or pre-enhancement process. Our results show that the developed device is capable of selectively detecting of cancer cells, within a concentrations range of 1000-100,000 cells/mL, with a detection limit of <1000 cells/mL, a response time of <5 min and sample volume of 50 μL of. The presented RIfS method shows considerable promise for translation to a rapid and cost-effective point-of-care diagnostic device for the detection of CTCs in patients with metastatic cancer.|
|Keywords:||Cell Line, Tumor; Humans; Aluminum Oxide; Cell Adhesion Molecules; Antibodies, Neoplasm; Antigens, Neoplasm; Microscopy, Electron, Scanning; Microfluidic Analytical Techniques; Spectrum Analysis; Equipment Design; Biosensing Techniques; Surface Properties; Porosity; Metal Nanoparticles; Neoplastic Cells, Circulating; Antibodies, Immobilized; Lab-On-A-Chip Devices; Limit of Detection; Epithelial Cell Adhesion Molecule|
|Rights:||Crown Copyright © 2012 Published by Elsevier B.V. All rights reserved.|
|Appears in Collections:||Chemical Engineering publications|
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