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Type: Journal article
Title: Cloning of the cDNA and localization of the gene encoding human NRPB, a ubiquitously expressed, multidomain putative adapter protein
Author: Hooper, J.
Baker, E.
Ogbourne, S.
Sutherland, G.
Antalis, T.
Citation: Genomics, 2000; 66(1):113-118
Publisher: Academic Press Inc
Issue Date: 2000
ISSN: 0888-7543
Abstract: Adapter proteins modulate multiple signaling pathways by regulating the aggregation of other factors into signaling complexes. Here we have identified a novel human cDNA encoding NRBP, a multidomain putative adapter protein containing (i) two putative nuclear receptor binding motifs (LXXLL), (ii) a putative binding domain for Src homology-2 (SH2) domain containing proteins, (iii) a kinase-like domain, (iv) a bipartite nuclear localization signal, and (v) three sequences rich in glutamic acid, serine, proline, and threonine (PEST) residues. The NRBP mRNA transcript, of approximately 2.4 kb, was ubiquitously expressed in a wide range of normal human tissues and 15 human tumor cell lines. The NRBP cDNA is predicted to encode a polypeptide of 535 amino acids with a molecular mass of 59.8 kDa. Translation of NRBP mRNA in vitro reveals three translation products of 60, 51, and 43 kDa, suggesting that translation of NRBP may initiate at multiple sites. The NRBP gene was localized to human chromosome 2p23, near the location of the NCOA1 gene encoding the nuclear receptor coactivator, steroid receptor coactivator-1 (SRC-1). The features of NRBP predict a function as an adapter protein potentially linking signaling pathways involving nuclear receptors and SH2 domain containing proteins.
Keywords: Chromosomes, Human, Pair 2; Humans; Macromolecular Substances; Phosphotransferases; Protein Kinases; Protein Sorting Signals; Vesicular Transport Proteins; Receptors, Cytoplasmic and Nuclear; DNA, Complementary; RNA, Messenger; 3' Untranslated Regions; 5' Untranslated Regions; Biological Factors; Chromosome Mapping; Cloning, Molecular; Signal Transduction; Binding Sites; Amino Acid Motifs; Base Sequence; src Homology Domains; Protein Binding; Sequence Homology, Amino Acid; Tissue Distribution; Open Reading Frames; Molecular Sequence Data
RMID: 0001000231
DOI: 10.1006/geno.2000.6167
Appears in Collections:Paediatrics publications

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