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Type: Journal article
Title: Use of Matrigel in culture affects cell phenotype and gene expression in the first trimester trophoblast cell line HTR8/SVneo
Author: Highet, A.
Zhang, V.
Heinemann, G.
Roberts, C.
Citation: Placenta, 2012; 33(7):586-588
Publisher: W B Saunders Co Ltd
Issue Date: 2012
ISSN: 0143-4004
Statement of
A.R. Highet, V.J. Zhang, G.K. Heinemann and C.T. Roberts
Abstract: There is inconsistent use of Matrigel for experiments with the HTR8/SVneo first trimester trophoblast and other cell lines. We quantified the effects of Matrigel on the expression of genes considered to be markers of extravillous cytotrophoblast (EVT) differentiation and invasive potential. Culture on Matrigel promoted formation of "endothelial-like" tubes and reduced mRNA expression of matrix metalloproteinase 2 (MMP2), cytokeratin 7 (KRT7) and integrin alpha 1 (ITGA1), while increasing VE-cadherin (CDH5) expression consistent with a vascular phenotype. This process may constitute part of the endothelial cell mimicry exhibited by endovascular EVTs invading the maternal spiral arteries. HTR8/SVneo appears to be phenotypically polymorphic and adopt endovascular morphology on Matrigel.
Keywords: Cell Line; Endothelial Cells; Trophoblasts; Humans; Collagen; Plastics; Proteoglycans; Laminin; Integrin alpha1; Drug Combinations; Cell Differentiation; Gene Expression; Gestational Age; Phenotype; Keratins; Matrix Metalloproteinase 2
Rights: © 2012 Elsevier Ltd. All rights reserved.
RMID: 0020119424
DOI: 10.1016/j.placenta.2012.04.003
Grant ID:
Appears in Collections:Paediatrics publications

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