Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/72968
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Type: Journal article
Title: Magnetic silica spheres with large nanopores for nucleic acid adsorption and cellular uptake
Author: Liu, J.
Wang, B.
Hartono, S.
Liu, T.
Kantharidis, P.
Middelberg, A.
Lu, G.
He, L.
Qiao, S.
Citation: Biomaterials, 2012; 33(3):970-978
Publisher: Elsevier Sci Ltd
Issue Date: 2012
ISSN: 0142-9612
1878-5905
Statement of
Responsibility: 
Jian Liu, Bo Wang, Sandy Budi Hartono, Tingting Liu, Phillip Kantharidis, Anton P.J. Middelberg, Gao Qing (Max) Lu, Lizhong He, Shi Zhang Qiao
Abstract: Template assisted fabrication of magnetic silica nanospheres with large nanopores (MSNLP) and their adsorption and delivery of nucleic acids are reported in this paper. Silica spheres with controlled particle diameter (~400 nm) and large nanopore size (13-24 nm) are prepared by using Brij56 as a template of mesopore, enabling incorporation of magnetic nanocrystals into the particles under mild neutral synthesis conditions. High resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), and field-dependent magnetisation measurements confirm that the magnetic nanocrystals have been encapsulated into the silica spheres. The saturation magnetisation values of the resulted magnetic-silica nanocomposites are tunable by adjusting the amount of Fe(3)O(4) magnetic nanocrystals used in the synthesis process. The nitrogen sorption analysis reveals that mesopores with large pore size exist in the silica matrix. After functionalisation of the silica surface with poly-(l-lysine) (PLL), the nanoparticles show strong adsorption capacity (q(m) ranging from 10 to 22.5 μg/mg) for CpG DNA. We have further demonstrated successful delivery of miRNA into rat proximal tubular epithelial cells, facilitated by efficient cellular uptake of the nanocomposites. This work provides a convenient strategy to prepare MSNLP which can offer a versatile platform for biological applications such as simultaneous drug delivery and magnetic resonance imagining under external magnetic field.
Keywords: Magnetic-silica nanocomposites
Mesoporous materials
Cellular uptake
CpG DNA adsorption
Large nanopores
Rights: Copyright © 2011 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.biomaterials.2011.10.001
Published version: http://dx.doi.org/10.1016/j.biomaterials.2011.10.001
Appears in Collections:Aurora harvest 5
Chemical Engineering publications

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