Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/73013
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | McAllister, L. | - |
dc.contributor.author | Ogunniyi, A. | - |
dc.contributor.author | Stroeher, U. | - |
dc.contributor.author | Paton, J. | - |
dc.contributor.editor | Chakravortty, D. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | PLoS One, 2012; 7(2):1-9 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/73013 | - |
dc.description.abstract | Streptococcus pneumoniae (the pneumococcus) is a formidable human pathogen, responsible for massive global morbidity and mortality. The ability to utilize carbohydrates in a variety of host niches appears to be integral to pneumococcal pathogenesis. In this study we investigated a genomic island, which includes a ROK family protein, a putative cellobiose phosphotransferase system (PTS) and a putative sulfatase. This accessory region is widespread in the pneumococcus in strains of various serotypes and levels of virulence. We have performed simple bioinformatic analysis of the region and investigated its role in vivo in 2 strains with markedly different virulence profiles (WCH206 of serotype 3, ST180; Menzies5 of serotype 11A, ST662). Deleting and replacing the entire island with an antibiotic resistance cassette caused the virulent serotype 3 strain to become attenuated in a murine pneumonia/sepsis model. Further mutants were constructed and used to show that various components of the island contribute significantly to the fitness of WCH206 in a variety of niches of this model, including the nasopharynx, ears and blood, but especially in the lungs. In addition, the island conferred a competitive advantage in nasopharyngeal colonization for the serotype 11A strain, which was essentially avirulent in the pneumonia/sepsis model. The contribution of this island to both pathogenesis and colonization may explain why this accessory region is widespread in the pneumococcus. | - |
dc.description.statementofresponsibility | Lauren J. McAllister, Abiodun D. Ogunniyi, Uwe H. Stroeher and James C. Paton | - |
dc.language.iso | en | - |
dc.publisher | Public Library of Science | - |
dc.rights | Copyright: © 2012 McAllister et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.1371/journal.pone.0032385 | - |
dc.subject | Nasopharynx | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Mice | - |
dc.subject | Streptococcus pneumoniae | - |
dc.subject | Sepsis | - |
dc.subject | Pneumonia | - |
dc.subject | Disease Models, Animal | - |
dc.subject | Phosphoenolpyruvate Sugar Phosphotransferase System | - |
dc.subject | DNA Mutational Analysis | - |
dc.subject | Computational Biology | - |
dc.subject | Virulence | - |
dc.subject | Gene Expression Regulation, Bacterial | - |
dc.subject | Mutagenesis | - |
dc.subject | Genomic Islands | - |
dc.subject | Mutation | - |
dc.subject | Genes, Bacterial | - |
dc.subject | Genome, Bacterial | - |
dc.subject | Genetic Fitness | - |
dc.title | Contribution of a genomic accessory region encoding a putative cellobiose phosphotransferase system to virulence of Streptococcus Pneumoniae | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1371/journal.pone.0032385 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Ogunniyi, A. [0000-0001-9308-5629] | - |
dc.identifier.orcid | Paton, J. [0000-0001-9807-5278] | - |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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hdl_73013.pdf | Published version | 665.88 kB | Adobe PDF | View/Open |
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