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Type: Journal article
Title: Biological efficacy and stability of diluted ticarcillin-clavulanic acid in the topical treatment of Pseudomonas aeruginosa infections
Author: Bateman, F.
Moss, S.
Trott, D.
Shipstone, M.
Citation: Veterinary Dermatology: an international journal, 2012; 23(2):23-29
Publisher: Blackwell Science Ltd
Issue Date: 2012
ISSN: 0959-4493
Statement of
Fiona L. Bateman, Susan M. Moss, Darren J. Trott, and Michael A. Shipstone
Abstract: Topical compounded Timentin(®) diluted with an inactive vehicle has been reported to be effective in the treatment of otitis externa caused by Pseudomonas aeruginosa. The aims of this study were to determine the biological efficacy of Timentin(®) (ticarcillin and clavulanic acid) when diluted in the carrier vehicle Methopt(®) against P. aeruginosa and to determine the efficacy and stability of Timentin(®) aqueous stock concentrate solution. Timentin(®) stock concentrate was tested against four P. aeruginosa isolates on days 0, 7, 14, 21 and 28; then after 2, 3, 4, 5, 6, 9 and 12 months of storage at 4 or -20°C. The diluted Timentin(®)-Methopt(®) solutions were tested against all isolates after 0, 2, 4, 6, 8, 10, 12, 14, 17, 21, 24 and 28 days of storage at 24 or 4°C. Minimal inhibitory concentration (MIC) levels for all strains were determined using the broth microdilution method. The MIC of the stock solution remained relatively constant and acceptable throughout the study when stored at -20°C and was also acceptable for shorter time periods (6-9 months) when stored at 4°C. The MIC for the diluted Timentin(®)-Methopt(®) solution remained relatively constant and acceptable throughout the study for all four bacterial strains, with no difference between the solutions stored at 4 or 24°C. The results of this study indicate that storage of the Timentin(®) stock solution at -20°C does not compromise efficacy for at least 12 months and that Timentin(®) diluted in Methopt(®) was stable for 28 days when stored at either 4 or 24°C.
Keywords: Animals
Pseudomonas aeruginosa
Clavulanic Acids
Anti-Bacterial Agents
Microbial Sensitivity Tests
Administration, Topical
Drug Storage
Chemistry, Pharmaceutical
Rights: © 2011 The Authors.
DOI: 10.1111/j.1365-3164.2011.01018.x
Published version:
Appears in Collections:Animal and Veterinary Sciences publications
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