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|Title:||Helicobacter-induced gastritis in mice not expressing metallothionein-I and II|
van den Berg, M.
van der Pas, M.
|Citation:||Helicobacter (Oxford), 2003; 8(5):533-541|
|Publisher:||Blackwell Publishing Inc|
|Cuong D. Tran, Hien Huynh, Maartje van den Berg, Mechtelt van der Pas, M. A. Fiona Campbell, Jeffrey C. Philcox, Peter Coyle, Allau M. Rofe and Ross N. Butler|
|Abstract:||BACKGROUND:Helicobacter pylori a primary cause of gastritis and peptic ulcer disease, is associated with increased production of reactive oxygen species within the gastric mucosa. Metallothionein (MT), a low-molecular-weight, cysteine-rich, metal-binding ligand, has been shown to sequester reactive oxygen species and reduce tissue damage. This study investigates the role of MT in H. pylori-induced gastritis in mice. MATERIALS AND METHODS:Control (MT+/+) and MT-null (MT-/-) mice were inoculated with either 1 x 108H. pylori or H. felis, and were infected for 4, 8 and 16 weeks or 8 weeks, respectively. H. pylori load was determined by culture. Myloperoxidase activity and MT levels were also determined. RESULTS:The stomachs of H. felis-infected mice were more severely inflamed than those of H. pylori-infected mice. H. felis-induced gastritis was more severe (p =.003) in MT-/- than in MT+/+ mice. MT-/- mice also had higher (60%; p <.05) H. pylori loads than MT+/+ mice 4 weeks after infection but not 8 or 16 weeks after infection. Myloperoxidase activity with H. pylori was similar between MT+/+ and MT-/- mice. Thirty-three per cent greater (p <.05) myloperoxidase activity was observed in MT-/- than in MT+/+ mice infected with H. felis. In MT+/+ mice infected with H. pylori, liver MT was increased by 33 and 39% (p <.05) at 8 and 16 weeks, respectively, whereas gastric MT increased by 46% (p <.05) at 4 weeks and declined to baseline levels at 8 and 16 weeks. CONCLUSIONS:Mice lacking MT are more susceptible to H. pylori colonization and gastric inflammation, indicating that MT may be protective against H. pylori-induced gastritis.|
Reactive Oxygen Species
Colony Count, Microbial
|Rights:||© 2003 Blackwell Publishing Ltd|
|Appears in Collections:||Aurora harvest|
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