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Type: Journal article
Title: Helicobacter-induced gastritis in mice not expressing metallothionein-I and II
Author: Tran, C.
Huynh, H.
van den Berg, M.
van der Pas, M.
Campbell, M.
Philcox, J.
Coyle, P.
Rofe, A.
Butler, R.
Citation: Helicobacter (Oxford), 2003; 8(5):533-541
Publisher: Blackwell Publishing Inc
Issue Date: 2003
ISSN: 1083-4389
Statement of
Cuong D. Tran, Hien Huynh, Maartje van den Berg, Mechtelt van der Pas, M. A. Fiona Campbell, Jeffrey C. Philcox, Peter Coyle, Allau M. Rofe and Ross N. Butler
Abstract: BACKGROUND:Helicobacter pylori a primary cause of gastritis and peptic ulcer disease, is associated with increased production of reactive oxygen species within the gastric mucosa. Metallothionein (MT), a low-molecular-weight, cysteine-rich, metal-binding ligand, has been shown to sequester reactive oxygen species and reduce tissue damage. This study investigates the role of MT in H. pylori-induced gastritis in mice. MATERIALS AND METHODS:Control (MT+/+) and MT-null (MT-/-) mice were inoculated with either 1 x 108H. pylori or H. felis, and were infected for 4, 8 and 16 weeks or 8 weeks, respectively. H. pylori load was determined by culture. Myloperoxidase activity and MT levels were also determined. RESULTS:The stomachs of H. felis-infected mice were more severely inflamed than those of H. pylori-infected mice. H. felis-induced gastritis was more severe (p =.003) in MT-/- than in MT+/+ mice. MT-/- mice also had higher (60%; p <.05) H. pylori loads than MT+/+ mice 4 weeks after infection but not 8 or 16 weeks after infection. Myloperoxidase activity with H. pylori was similar between MT+/+ and MT-/- mice. Thirty-three per cent greater (p <.05) myloperoxidase activity was observed in MT-/- than in MT+/+ mice infected with H. felis. In MT+/+ mice infected with H. pylori, liver MT was increased by 33 and 39% (p <.05) at 8 and 16 weeks, respectively, whereas gastric MT increased by 46% (p <.05) at 4 weeks and declined to baseline levels at 8 and 16 weeks. CONCLUSIONS:Mice lacking MT are more susceptible to H. pylori colonization and gastric inflammation, indicating that MT may be protective against H. pylori-induced gastritis.
Keywords: Stomach
Gastric Mucosa
Mice, Knockout
Helicobacter felis
Helicobacter pylori
Helicobacter Infections
Reactive Oxygen Species
Colony Count, Microbial
Rights: © 2003 Blackwell Publishing Ltd
DOI: 10.1046/j.1523-5378.2003.00174.x
Appears in Collections:Aurora harvest
Paediatrics publications

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