Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/73251
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Type: Journal article
Title: A comparison of hydroponic and soil-based screening methods to identify salt tolerance in the field in barley
Author: Tavakkoli, E.
Fatehi, F.
Rengasamy, P.
McDonald, G.
Citation: Journal of Experimental Botany, 2012; 63(10):3853-3868
Publisher: Oxford Univ Press
Issue Date: 2012
ISSN: 0022-0957
1460-2431
Statement of
Responsibility: 
Ehsan Tavakkoli, Foad Fatehi, Pichu Rengasamy and Glenn K. McDonald
Abstract: Success in breeding crops for yield and other quantitative traits depends on the use of methods to evaluate genotypes accurately under field conditions. Although many screening criteria have been suggested to distinguish between genotypes for their salt tolerance under controlled environmental conditions, there is a need to test these criteria in the field. In this study, the salt tolerance, ion concentrations, and accumulation of compatible solutes of genotypes of barley with a range of putative salt tolerance were investigated using three growing conditions (hydroponics, soil in pots, and natural saline field). Initially, 60 genotypes of barley were screened for their salt tolerance and uptake of Na+, Cl–, and K+ at 150 mM NaCl and, based on this, a subset of 15 genotypes was selected for testing in pots and in the field. Expression of salt tolerance in saline solution culture was not a reliable indicator of the differences in salt tolerance between barley plants that were evident in saline soil-based comparisons. Significant correlations were observed in the rankings of genotypes on the basis of their grain yield production at a moderately saline field site and their relative shoot growth in pots at ECe 7.2 [Spearman’s rank correlation (rs)=0.79] and ECe 15.3 (rs=0.82) and the crucial parameter of leaf Na+ (rs=0.72) and Cl– (rs=0.82) concentrations at ECe 7.2 dS m−1. This work has established screening procedures that correlated well with grain yield at sites with moderate levels of soil salinity. This study also showed that both salt exclusion and osmotic tolerance are involved in salt tolerance and that the relative importance of these traits may differ with the severity of the salt stress. In soil, ion exclusion tended to be more important at low to moderate levels of stress but osmotic stress became more important at higher stress levels. Salt exclusion coupled with a synthesis of organic solutes were shown to be important components of salt tolerance in the tolerant genotypes and further field tests of these plants under stress conditions will help to verify their potential utility in crop-improvement programmes.
Keywords: Barley; hydroponics; osmotic stress; physiological traits; salinity tolerance; screening; soil; specific ion toxicity.
Rights: © 2012 The Author(s).This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
RMID: 0020120724
DOI: 10.1093/jxb/ers085
Appears in Collections:Agriculture, Food and Wine publications

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