Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/73371
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | May, K. | - |
dc.contributor.author | Grabowicz, M. | - |
dc.contributor.author | Polyak, S. | - |
dc.contributor.author | Morona, R. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Microbiology, 2012; 158(7):1874-1883 | - |
dc.identifier.issn | 1350-0872 | - |
dc.identifier.issn | 1465-2080 | - |
dc.identifier.uri | http://hdl.handle.net/2440/73371 | - |
dc.description.abstract | The IcsA autotransporter protein is a major virulence factor of the human intracellular pathogen Shigella flexneri. IcsA is distributed at the poles in the outer membrane (OM) of S. flexneri and interacts with components of the host actin-polymerization machinery to facilitate intracellular actin-based motility and subsequent cell-to-cell spreading of the bacterium. We sought to characterize the biochemical properties of IcsA in the bacterial OM. Chemical cross-linking data suggested that IcsA exists in a complex in the OM. Furthermore, reciprocal co-immunoprecipitation of differentially epitope-tagged IcsA proteins indicated that IcsA is able to self-associate. The identification of IcsA linker-insertion mutants that were negatively dominant provided genetic evidence of IcsA–IcsA interactions. From these results, we propose a model whereby IcsA self-association facilitates efficient actin-based motility. | - |
dc.description.statementofresponsibility | Kerrie L. May, Marcin Grabowicz, Steven W. Polyak and Renato Morona | - |
dc.language.iso | en | - |
dc.publisher | Soc General Microbiology | - |
dc.relation.isreplacedby | 2440/90094 | - |
dc.relation.isreplacedby | http://hdl.handle.net/2440/90094 | - |
dc.rights | © 2012 SGM | - |
dc.source.uri | http://dx.doi.org/10.1099/mic.0.056465-0 | - |
dc.subject | Shigella flexneri | - |
dc.subject | Bacterial Proteins | - |
dc.subject | Bacterial Outer Membrane Proteins | - |
dc.subject | DNA-Binding Proteins | - |
dc.subject | Transcription Factors | - |
dc.subject | Virulence Factors | - |
dc.subject | Cross-Linking Reagents | - |
dc.subject | Immunoprecipitation | - |
dc.subject | Protein Binding | - |
dc.subject | Protein Multimerization | - |
dc.title | Self-association of the Shigella flexneri IcsA autotransporter protein | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1099/mic.0.056465-0 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Polyak, S. [0000-0002-8458-5194] | - |
dc.identifier.orcid | Morona, R. [0000-0001-7009-7440] | - |
Appears in Collections: | Aurora harvest 7 IPAS publications Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_73371.pdf | Accepted version | 7.61 MB | Adobe PDF | View/Open |
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