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Type: Journal article
Title: Isolation of antigen-specific B cells
Author: Kodituwakku, A.
Jessup, C.
Zola, H.
Roberton, D.
Citation: Immunology and Cell Biology, 2003; 81(3):163-170
Publisher: Blackwell Publishing Asia
Issue Date: 2003
ISSN: 0818-9641
Abstract: Cell separation techniques are important in immunology. Major cell populations can be separated successfully with high purity. However, isolation of cells which are specific for particular antigens is more challenging because of the relatively small numbers of antigen-specific cells, and the lack of independent markers available to determine the purity of the isolated population. In this review, the literature describing three principal techniques used to separate antigen-specific cells has been reviewed. Particular emphasis has been placed on yield and purity; the two most important parameters of any purification method. The most promising isolation methods have used immunomagnetic sorting and multiparametric flow cytometric analysis.
Keywords: Antigen-specific B cell; cell isolation; flow cytometry; immunomagnetic separation
RMID: 0020032101
DOI: 10.1046/j.1440-1711.2003.01152.x
Appears in Collections:Paediatrics publications

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