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https://hdl.handle.net/2440/7395
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Type: | Journal article |
Title: | Bacterial lipopolysaccharide and tumor necrosis factor alpha synergistically increase expression of human endothelial adhesion molecules through activation of NF-kB and p38 mitogen-activated protein kinase signaling pathways |
Author: | Jersmann, H. Hii, C. Ferrante, J. Ferrante, A. |
Citation: | Infection and Immunity, 2001; 69(3):1273-1279 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2001 |
ISSN: | 0019-9567 1098-5522 |
Editor: | Moore, R.N. |
Statement of Responsibility: | Hubertus P. A. Jersmann, Charles S. T. Hii, Judith V. Ferrante, and Antonio Ferrante |
Abstract: | One of the recognized associations of bacterial infection with cardiovascular events is the activation of endothelium and upregulation of adhesion molecules. The two major proinflammatory mediators implicated in the causation of cardiovascular events, bacterial lipopolysaccharide (LPS) and tumor necrosis factor alpha (TNF), were found to cooperate to enhance the adhesive properties of endothelial cells. These caused synergistic upregulation of intercellular adhesion molecule-1, E-selectin, and vascular cell adhesion molecule-1 in human umbilical vein endothelial cells as determined by flow cytometry analysis and enzyme-linked immunosorbent assay. This synergism was not due to TNF causing an upregulation of CD14 expression. Treatment with both LPS and TNF resulted in a marked increase in the translocation of NF-B into the nucleus. The activity of p38 mitogen-activated protein kinase was also synergistically enhanced, while the activity of c-jun N-terminal kinase was increased in an additive manner. The results demonstrate that LPS and TNF act synergistically to upregulate the expression of endothelial cell adhesion molecules, possibly by amplification of signaling pathways upstream of transcription. These findings have implications for the understanding of the acceleration of atherosclerotic events seen in low-grade infections with gram-negative organisms. |
Keywords: | Endothelium, Vascular Cell Nucleus Humans Mitogen-Activated Protein Kinases p38 Mitogen-Activated Protein Kinases Lipopolysaccharides Tumor Necrosis Factor-alpha NF-kappa B Cell Adhesion Molecules Intercellular Adhesion Molecule-1 E-Selectin Vascular Cell Adhesion Molecule-1 Cell Compartmentation Up-Regulation Biological Transport Drug Synergism |
Description: | Copyright © 2001, American Society for Microbiology. All rights reserved. |
DOI: | 10.1128/IAI.69.3.1273-1279.2001 |
Published version: | http://dx.doi.org/10.1128/iai.69.3.1273-1279.2001 |
Appears in Collections: | Aurora harvest 5 Paediatrics publications |
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