Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/73978
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DC Field | Value | Language |
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dc.contributor.author | Tu, W. | - |
dc.contributor.author | Muhlhausler, B. | - |
dc.contributor.author | James, M. | - |
dc.contributor.author | Stone, D. | - |
dc.contributor.author | Gibson, R. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, 2012; 423(1):176-182 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.uri | http://hdl.handle.net/2440/73978 | - |
dc.description.abstract | Desaturase and elongase are two key enzyme categories in the long-chain polyunsaturated fatty acid (LCPUFA) pathway that convert dietary α-linolenic acid (18:3n-3) to docosahexaenoic acid (22:6n-3). The Δ6 desaturase is considered as rate limiting in the conversion. In a previous study in barramundi we demonstrated that the desaturase had a low Δ6 activity but noted that the enzyme also possessed Δ8 ability that utilised 20-carbon fatty acids. This observation suggests that an alternative pathway may exist in the barramundi via elongases to form 20-carbon metabolites from 18:3n-3 to 20:3n-3 and then Δ6/8 desaturase to 20:4n-3. Cloning of the barramundi elongation of very long-chain fatty acid gene (ELOVL) and heterologous expression of the corresponding elongase were performed to examine activity with regard to time course, substrate concentration and substrate preference. Results revealed that the barramundi elongase showed a broad range of substrate specificity including 18-carbon PUFA (including 18:3n-3 and 18:2n-6), 20- and 22-carbon LCPUFA, with greater activity towards omega-3 (n-3) than n-6 fatty acids. The findings from this study provide molecular evidence for an alternative n-3 fatty acid elongation pathway utilising 18:3n-3 in barramundi. | - |
dc.description.statementofresponsibility | Wei-Chun Tu, Beverly S. Mühlhäusler, Michael J. James, David A. J. Stone, Robert A. Gibson | - |
dc.language.iso | en | - |
dc.publisher | Academic Press Inc | - |
dc.rights | © 2012 Elsevier Inc. All rights reserved. | - |
dc.source.uri | http://dx.doi.org/10.1016/j.bbrc.2012.05.110 | - |
dc.subject | α-Linolenic acid (18:3n-3) | - |
dc.subject | Elongase | - |
dc.subject | ELOVL | - |
dc.subject | Heterologous expression | - |
dc.subject | LCPUFA metabolism | - |
dc.title | An alternative n-3 fatty acid elongation pathway utilising 18:3n-3 in barramundi (Lates calcarifer) | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/j.bbrc.2012.05.110 | - |
dc.relation.grant | ARC | - |
dc.relation.grant | NHMRC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Muhlhausler, B. [0000-0002-9021-6790] | - |
dc.identifier.orcid | James, M. [0000-0002-4918-2998] | - |
dc.identifier.orcid | Gibson, R. [0000-0002-8750-525X] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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hdl_73978.pdf | Submitted version | 929.49 kB | Adobe PDF | View/Open |
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