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Type: Journal article
Title: Functional characterization of the rice SPX-MFS family reveals a key role of OsSPX-MFS1 in controlling phosphate homeostasis in leaves
Author: Wang, C.
Huang, W.
Ying, Y.
Li, S.
Secco, D.
Tyerman, S.
Whelan, J.
Shou, H.
Citation: New Phytologist, 2012; 196(1):139-148
Publisher: Blackwell Publishing Ltd
Issue Date: 2012
ISSN: 0028-646X
Statement of
Chuang Wang, Wei Huang, Yinghui Ying, Shuai Li, David Secco, Steve Tyerman, James Whelan and Huixia Shou
Abstract: Proteins possessing the SPX domain are found in several proteins involved in inorganic phosphate (Pi) transport and signalling in yeast and plants. Although the functions of several SPX-domain protein subfamilies have recently been uncovered, the role of the SPX-MFS subfamily is still unclear. Using quantitative RT-PCR analysis, we studied the regulation of SPX-MFS gene expression by the central regulator, OsPHR2 and Pi starvation. The function of OsSPX-MFS1 in Pi homeostasis was analysed using an OsSPX-MFS1 mutant (mfs1) and osa-miR827 overexpression line (miR827-Oe). Finally, heterologous complementation of a yeast mutant impaired in Pi transporter was used to assess the capacity of OsSPX-MFS1 to transport Pi. Transcript analyses revealed that members of the SPX-MFS family were mainly expressed in the shoots, with OsSPX-MFS1 and OsSPX-MFS3 being suppressed by Pi deficiency, while OsSPX-MFS2 was induced. Mutation in OsSPX-MFS1 (mfs1) and overexpression of the upstream miR827 (miR827-Oe) plants impaired Pi homeostasis in the leaves. In addition, studies in yeast revealed that OsSPX-MFS1 may be involved in Pi transport. The results suggest that OsSPX-MFS1 is a key player in maintaining Pi homeostasis in the leaves, potentially acting as a Pi transporter.
Keywords: Homeostasis; miRNA; OsPHR2; phosphate; rice; SPX; transporter
Rights: © 2012 The Authors. New Phytologist © 2012 New Phytologist Trust
RMID: 0020121797
DOI: 10.1111/j.1469-8137.2012.04227.x
Appears in Collections:Agriculture, Food and Wine publications

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