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Type: Journal article
Title: Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45)
Author: Rajasekariah, P.
Eyre, H.
Stanley, K.
Walls, R.
Sutherland, G.
Citation: International Journal of Biochemistry and Cell Biology, 1999; 31(6):683-694
Issue Date: 1999
ISSN: 1357-2725
Statement of
Poornima Rajasekariah, Helen J Eyre, Keith K Stanley, Ronald S Walls, Grant R Sutherland
Abstract: We have isolated a novel cDNA clone from human leucocyte cDNA library, encoding a Sec1p-like vacuolar protein sorting (hlVps45) which is believed to be implicated in vesicular transportation. Although the deduced amino acid (AA) sequence of this cDNA has revealed 97% identity to other known mammalian vacuolar protein sorting, there is an extensive variation in nucleotide sequence in comparison to that of three previously reported human (hVps45), rat (rVps45) and mouse (mVps45) vacuolar protein sorting (Vps45) cDNAs [ [1], [2] and [3]]. At the nucleotide sequence level hlVps45 demonstrated 90% homology to the hVps45 and rVps45 and 89% identity to mVps45 with no significant homology in their noncoding regions. The 2.4 Kb mRNA corresponding to the hlVps45 clone is widely distributed in a variety of human tissues expressing highest levels in peripheral blood mononuclear cells (PBMC), neutrophils, heart, spleen, and testis. The chromosomal mapping studies have demonstrated that the hlVps45 is localized to long arm of human chromosome 1 at q21–q22. Our data indicates that we have isolated, characterized and mapped a novel cDNA encoding hlVps45, which may play an important role in protein trafficking as well as have clinical significance in the release of inflammatory mediators e.g. histamine, bradykinin and cytokine release.
Keywords: Leukocytes; Chromosomes, Human, Pair 1; Golgi Apparatus; Humans; Carrier Proteins; Membrane Proteins; Vesicular Transport Proteins; DNA, Complementary; RNA, Messenger; Chromosome Mapping; Cloning, Molecular; Reverse Transcriptase Polymerase Chain Reaction; Sequence Alignment; Amino Acid Sequence; Base Sequence; Biological Transport; Molecular Sequence Data
RMID: 0030005573
DOI: 10.1016/S1357-2725(99)00017-5
Appears in Collections:Paediatrics publications

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