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Type: Journal article
Title: Immobilized Streptavidin gradients as bioconjugation platforms
Author: Coad, B.
Vasilev, K.
Diener, K.
Hayball, J.
Short, R.
Griesser, H.
Citation: Langmuir, 2012; 28(5):2710-2717
Publisher: Amer Chemical Soc
Issue Date: 2012
ISSN: 0743-7463
Statement of
Bryan R. Coad, Krasimir Vasilev, Kerrilyn R. Diener, John D. Hayball, Robert D. Short and Hans J. Griesser
Abstract: Surface density gradients of streptavidin (SAV) were created on solid surfaces and demonstrated functionality as a bioconjugation platform. The surface density of immobilized streptavidin steadily increased in one dimension from 0 to 235 ng cm(-2) over a distance of 10 mm. The density of coupled protein was controlled by its immobilization onto a polymer surface bearing a gradient of aldehyde group density, onto which SAV was covalently linked using spontaneous imine bond formation between surface aldehyde functional groups and primary amine groups on the protein. As a control, human serum albumin was immobilized in the same manner. The gradient density of aldehyde groups was created using a method of simultaneous plasma copolymerization of ethanol and propionaldehyde. Control over the surface density of aldehyde groups was achieved by manipulating the flow rates of these vapors while moving a mask across substrates during plasma discharge. Immobilized SAV was able to bind biotinylated probes, indicating that the protein retained its functionality after being immobilized. This plasma polymerization technique conveniently allows virtually any substrate to be equipped with tunable protein gradients and provides a widely applicable method for bioconjugation to study effects arising from controllable surface densities of proteins.
Keywords: Humans; Biotin; Polymers; Serum Albumin; Streptavidin; Biotinylation; Surface Properties
Rights: Copyright © 2012 American Chemical Society
RMID: 0020117091
DOI: 10.1021/la204714p
Appears in Collections:Obstetrics and Gynaecology publications

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