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|Title:||Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni|
Rodriguez Lopez, C.
|Citation:||Nature Communications, 2011; 2(article 424):1-10|
|Publisher:||Nature Publishing Group|
|Kathrin K. Geyer, Carlos M. Rodríguez López, Iain W. Chalmers, Sabrina E. Munshi, Martha Truscott, James Heald, Mike J. Wilkinson and Karl F. Hoffmann|
|Abstract:||Similar to other metazoan pathogens, Schistosoma mansoni undergoes transcriptional and developmental regulation during its complex lifecycle and host interactions. DNA methylation as a mechanism to control these processes has, to date, been discounted in this parasite. Here we show the first evidence for cytosine methylation in the S. mansoni genome. Transcriptional coregulation of novel DNA methyltransferase (SmDnmt2) and methyl-CpG-binding domain proteins mirrors the detection of cytosine methylation abundance and implicates the presence of a functional DNA methylation machinery. Genome losses in cytosine methylation upon SmDnmt2 silencing and the identification of a hypermethylated, repetitive intron within a predicted forkhead gene confirm this assertion. Importantly, disruption of egg production and egg maturation by 5-azacytidine establishes an essential role for 5-methylcytosine in this parasite. These findings provide the first functional confirmation for this epigenetic modification in any worm species and link the cytosine methylation machinery to platyhelminth oviposition processes.|
|Keywords:||Animals; Mice, Inbred C57BL; Humans; Mice; Schistosoma mansoni; Schistosomiasis mansoni; Cytosine; Helminth Proteins; Virulence; DNA Methylation; Oviposition; Female|
|Rights:||© 2011 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.|
|Appears in Collections:||Agriculture, Food and Wine publications|
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