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https://hdl.handle.net/2440/7425
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Type: | Journal article |
Title: | IgE+ cells in the peripheral blood of atopic, non-atopic and bee-venom hypersensitive individuals exhibit the phenotype of highly-differentiated B cells |
Author: | Donohoe, P. Heddle, R. Sykes, P. Fusco, M. Flego, L. Zola, H. |
Citation: | Journal of Allergy and Clinical Immunology, 1995; 95(2):587-596 |
Publisher: | Mosby |
Issue Date: | 1995 |
ISSN: | 0091-6749 1097-6825 |
Statement of Responsibility: | Peter J. Donohoe, Robert J. Heddle, Pamela J. Sykes, Michael Fusco, Loretta R. Flego, and Heddy Zola |
Abstract: | We have analyzed IgE⁺ cells in peripheral blood of atopic donors, donors hypersensitive to bee venom, and nonatopic control donors with two- and three-color flow cytometry. Although the percentage of IgE⁺ cells varied among these groups, the overall phenotypic patterns were similar. Most IgE⁺ cells do not display typical B-cell markers, such as CD19, CD20, and CD21. A significant proportion of these cells stain for CD38, indicating that they are more differentiated. IgE⁺ cells express FcγRII and CD45RO, an isoform associated with an advanced level of differentiation. The majority of IgE⁺ cells do not coexpress other surface immunoglobulin isotypes. In the case of bee venom–hypersensitive donors, we have been able to identify a small population of IgE⁺ cells with a specificity for phospholipase A2, a major immunogenic component of bee venom. The phospholipase A2⁺ cells display a phenotype similar to that of the IgE+ cells. |
Keywords: | bee venom hypersensitivity desensitization enumeration IgE cell phenotype immunotherapy mechanism |
DOI: | 10.1016/S0091-6749(95)70321-7 |
Published version: | http://dx.doi.org/10.1016/s0091-6749(95)70321-7 |
Appears in Collections: | Aurora harvest Paediatrics publications |
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