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|Title:||IgE+ cells in the peripheral blood of atopic, non-atopic and bee-venom hypersensitive individuals exhibit the phenotype of highly-differentiated B cells|
|Citation:||Journal of Allergy and Clinical Immunology, 1995; 95(2):587-596|
|Peter J. Donohoe, Robert J. Heddle, Pamela J. Sykes, Michael Fusco, Loretta R. Flego, and Heddy Zola|
|Abstract:||We have analyzed IgE⁺ cells in peripheral blood of atopic donors, donors hypersensitive to bee venom, and nonatopic control donors with two- and three-color flow cytometry. Although the percentage of IgE⁺ cells varied among these groups, the overall phenotypic patterns were similar. Most IgE⁺ cells do not display typical B-cell markers, such as CD19, CD20, and CD21. A significant proportion of these cells stain for CD38, indicating that they are more differentiated. IgE⁺ cells express FcγRII and CD45RO, an isoform associated with an advanced level of differentiation. The majority of IgE⁺ cells do not coexpress other surface immunoglobulin isotypes. In the case of bee venom–hypersensitive donors, we have been able to identify a small population of IgE⁺ cells with a specificity for phospholipase A2, a major immunogenic component of bee venom. The phospholipase A2⁺ cells display a phenotype similar to that of the IgE+ cells.|
|Keywords:||bee venom hypersensitivity; desensitization; enumeration; IgE cell phenotype; immunotherapy mechanism|
|Appears in Collections:||Paediatrics publications|
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