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|Title:||Increased placental nutrient transporter expression at mid-gestation after maternal growth hormone treatment in pigs: A placental mechanism for increased fetal growth|
De Blasio, M.
Van Wettere, W.
|Citation:||Biology of Reproduction, 2012; online(5):1-17|
|Publisher:||Soc Study Reproduction|
|Tung E, Roberts CT, Heinemann GK, De Blasio MJ, Kind KL, van Wettere WH, Owens JA, Gatford KL.|
|Abstract:||Growth hormone (GH) is important in maternal adaptation to pregnancy, and maternal circulating GH concentrations are reduced in human growth-restricted pregnancies. In the pig, maternal GH treatment throughout early-mid pregnancy increases fetal growth, despite constraining effects of adolescent and primiparous pregnancy, high litter size and restricted maternal nutrition. Since GH cannot cross the placenta, and does not increase placental weight, we hypothesised that its effects on fetal growth might be via improved placental structure or function. We therefore investigated effects of maternal GH treatment in pigs on structural correlates of placental function and placental expression of nutrient transporters important to fetal growth. Multiparous (sows) and primiparous pregnant pigs (gilts) were treated with GH (~15 µg kg(-1) day(-1)) or vehicle from days 25-50 of gestation (n = 7-8 per group, term ~115 days). Placentas were collected at day 50 of gestation and we measured structural correlates of function and expression of SLC2A1 (previously known as GLUT1) and SLC38A2 (previously known as SNAT2) nutrient transporters. Maternal GH treatment did not alter placental size or structure, increased protein expression of SLC2A1 in trophoblast (+35%, P = 0.037) and on its basal membrane (+44%, P = 0.011), and increased SLC38A2 protein expression in the basal (+44%, P = 0.001), but not the apical cytoplasm of trophoblast. Our findings suggest that maternal GH treatment increases fetal growth, in part, by enhancing placental nutrient transporter protein expression and hence fetal nutrient supply and trophoblast proliferation and differentiation and may have potential to ameliorate intrauterine growth restriction.|
|Keywords:||Trophoblasts; Placenta; Animals; Sus scrofa; Fetal Weight; Growth Hormone; Receptor, IGF Type 1; Amino Acid Transport System A; Organ Size; Immunohistochemistry; Fetal Development; Gestational Age; Pregnancy; Female; Glucose Transporter Type 1|
|Rights:||Copyright status unknown|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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