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|Title:||Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers|
|Citation:||Journal of Clinical Immunology, 2002; 22(6):338-344|
|Publisher:||Kluwer Academic/plenum Publ|
|Joanna S. Hawkes, Dani-Louise Bryan and Robert A. Gibson|
|Abstract:||Samples of milk (n=80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1β, interleukin-6, and tumor necrosis factor-α than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40–50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1β, interleukin-6, and tumor necrosis factor-α in the mammary gland and have the capacity to respond to further stimulation after leaving the breast.|
|Keywords:||Human milk; human milk cells; peripheral blood mononuclear cells; interleukin-1β; interleukin-6; tumor necrosis factor-α|
|Rights:||© Springer, Part of Springer Science+Business Media|
|Appears in Collections:||Paediatrics publications|
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