Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/7463
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Cytokine production by human milk cells and peripheral blood mononuclear cells from the same mothers
Author: Hawkes, J.
Bryan, D.
Gibson, R.
Citation: Journal of Clinical Immunology, 2002; 22(6):338-344
Publisher: Kluwer Academic/plenum Publ
Issue Date: 2002
ISSN: 0271-9142
1573-2592
Statement of
Responsibility: 
Joanna S. Hawkes, Dani-Louise Bryan and Robert A. Gibson
Abstract: Samples of milk (n=80) and venous blood were collected at 5 weeks postpartum from 82 lactating mothers. Human milk cells and peripheral blood mononuclear cells were isolated and the production of interleukin-1β, interleukin-6, and tumor necrosis factor-α in the absence and presence of lipopolysaccharide was determined by enzyme-linked immunosorbent assay. Human milk cells spontaneously produced significantly less interleukin-1β, interleukin-6, and tumor necrosis factor-α than peripheral blood mononuclear cells in the absence of stimulation. In vitro stimulation of human milk cells with lipopolysaccharide (500 ng/ml) for 24 hr increased cytokine production by approximately 40–50%, whereas peripheral blood mononuclear cells responded to lipopolysaccharide (200 ng/ml) with increased cytokine production of up to 350%. These observations suggest that cells in milk are capable of active involvement in the production of the interleukin-1β, interleukin-6, and tumor necrosis factor-α in the mammary gland and have the capacity to respond to further stimulation after leaving the breast.
Keywords: Human milk; human milk cells; peripheral blood mononuclear cells; interleukin-1β; interleukin-6; tumor necrosis factor-α
Rights: © Springer, Part of Springer Science+Business Media
RMID: 0020020261
DOI: 10.1023/A:1020652215048
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.