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Type: Journal article
Title: A glutathione-based system for defense against carbonyl stress in Haemophilus influenzae
Author: Kidd, S.
Jiang, C.
Tikhomirova, A.
Jennings, M.
McEwan, A.
Citation: BMC Microbiology, 2012; 12(1):1-6
Publisher: BioMed Central Ltd.
Issue Date: 2012
ISSN: 1471-2180
Statement of
Stephen P Kidd, Donald Jiang, Alexandra Tikhomirova, Michael P Jennings and Alastair G McEwan
Abstract: BACKGROUND adhC from Haemophilus influenzae encodes a glutathione-dependent alcohol dehydrogenase that has previously been shown to be required for protection against killing by S-nitrosoglutathione (GSNO). This group of enzymes is known in other systems to be able to utilize substrates that form adducts with glutathione, such as aldehydes. RESULTS Here, we show that expression of adhC is maximally induced under conditions of high oxygen tension as well as specifically with glucose as a carbon source. adhC could also be induced in response to formaldehyde but not GSNO. An adhC mutant was more susceptible than wild-type Haemophilus influenzae Rd KW20 to killing by various short chain aliphatic aldehydes, all of which can be generated endogenously during cell metabolism but are also produced by the host as part of the innate immune response. CONCLUSIONS These results indicate that AdhC plays a role in defense against endogenously generated reactive carbonyl electrophiles in Haemophilus influenzae and may also play a role in defense against the host innate immune system.
Keywords: Stress response; H. influenzae; Reactive aldehydes
Rights: © 2012 Kidd et al.; licensee BioMed Central Ltd.
RMID: 0020123312
DOI: 10.1186/1471-2180-12-159
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Appears in Collections:Molecular and Biomedical Science publications

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