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Type: Journal article
Title: A combined 13CO2/H2 breath test can be used to assess starch digestion and fermentation in humans
Author: Symonds, E.
Kritas, S.
Omari, T.
Butler, R.
Citation: Journal of Nutrition, 2004; 134(5):1193-1196
Publisher: Amer Inst Nutrition
Issue Date: 2004
ISSN: 0022-3166
Abstract: Ingestion of starch from corn (naturally enriched with (13)C) should produce (13)CO(2) after small intestinal digestion and (13)CO(2) and H(2) from colonic fermentation. This study used a combined (13)CO(2)/H(2) breath test to assess the digestion and fermentation of resistant starch and to show that the test could detect changes in digestibility due to cooking. Volunteers consumed 40 g digestible cornstarch with water (n = 8), or 40 g resistant cornstarch in liquid (n = 12) or cooked into a pancake (n = 4). Interval breath sampling was performed and analyzed for (13)CO(2) and H(2). Ingestion of resistant starch produced a double-peaked (13)CO(2) excretion curve. The first increase in (13)CO(2) occurred at the same time as excretion from digestible starch (55 +/- 9 and 68 +/- 9 min, respectively), which was due to small intestinal digestion. The second increase in (13)CO(2) was accompanied by an increase in H(2) excretion (432 +/- 15 and 428 +/- 48 min, respectively), which was indicative of colonic bacterial fermentation. Cooking resistant starch increased its degree of digestion from 36 to 72%. The (13)CO(2)/H(2) breath test can be used to estimate digestion and fermentation of starches in different physiologic and pathologic conditions.
Keywords: Humans; Carbon Dioxide; Hydrogen; Carbon Isotopes; Starch; Breath Tests; Fermentation; Digestion; Adult; Female; Cooking
RMID: 0020040979
DOI: 10.1093/jn/134.5.1193
Appears in Collections:Paediatrics publications

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