Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/75773
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Type: Journal article
Title: Walnut oil increases cholesterol efflux through inhibition of stearoyl CoA desaturase 1 in THP-1 macrophage-derived foam cells
Author: Zhang, J.
Grieger, J.
Kris-Etherton, P.
Thompson, J.
Gillies, P.
Fleming, J.
Vanden Heuvel, J.
Citation: Nutrition & Metabolism, 2011; 8(61):1-13
Publisher: BioMed Central Ltd.
Issue Date: 2011
ISSN: 1743-7075
1743-7075
Statement of
Responsibility: 
Jun Zhang, Jessica A Grieger, Penny M Kris-Etherton, Jerry T Thompson, Peter J Gillies, Jennifer A Fleming and John P Vanden Heuvel
Abstract: Background: Walnuts significantly decrease total and low-density lipoprotein cholesterol in normo- and hypercholesterolemic individuals. No study to date has evaluated the effects of walnuts on cholesterol efflux, the initial step in reverse cholesterol transport, in macrophage-derived foam cells (MDFC). The present study was conducted to investigate the mechanisms by which walnut oil affects cholesterol efflux. Methods: The extract of English walnuts (walnut oil) was dissolved in DMSO and applied to cultured THP-1 MDFC cells (0.5 mg/mL). THP-1 MDFC also were treated with human sera (10%, v:v) taken from subjects in a walnut feeding study. Cholesterol efflux was examined by liquid scintillation counting. Changes in gene expression were quantified by real time PCR. Results: Walnut oil treatment significantly increased cholesterol efflux through decreasing the expression of the lipogenic enzyme stearoyl CoA desaturase 1 (SCD1) in MDFC. Alpha-linolenic acid (ALA), the major n-3 polyunsaturated fatty acids found in walnuts, recaptured SCD1 reduction in MDFC, a mechanism mediated through activation of nuclear receptor farnesoid-X-receptor (FXR). Postprandial serum treatment also increased cholesterol efflux in MDFC. When categorized by baseline C-reactive protein (CRP; cut point of 2 mg/L), subjects in the lower CRP sub-group benefited more from dietary intervention, including a more increase in cholesterol efflux, a greater reduction in SCD1, and a blunted postprandial lipemia. Conclusion: In conclusion, walnut oil contains bioactive molecules that significantly improve cholesterol efflux in MDFC. However, the beneficial effects of walnut intake may be reduced by the presence of a pro-inflammatory state.
Keywords: cholesterol efflux; CRP; FXR; SCD1; walnut oil
Description: Extent: 13p.
Rights: © 2011 Zhang et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0020122342
DOI: 10.1186/1743-7075-8-61
Appears in Collections:Molecular and Biomedical Science publications

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