Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/759
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Type: Journal article
Title: Design analysis for refolding monomeric protein
Author: Kotlarski, N.
O'Neill, B.
Francis, G.
Middelberg, A.
Citation: AIChE Journal, 1997; 43(8):2123-2132
Publisher: WILEY-BLACKWELL
Issue Date: 1997
ISSN: 0001-1541
1547-5905
Abstract: Renaturation of protein expressed as inclusion bodies within Escherichia coli is a key step in many bioprocesses. Operating conditions for the refolding step dramatically affect the amount of protein product recovered, and hence profoundly influence the process economics. The first systematic comparison of refolding conducted in batch, fed-batch and continuous stirred-tank reactors is provided. Refolding is modeled as kinetic competition between first-order refolding (equilibrium reaction) and irreversible aggregation (second-order). Simulations presented allow direct comparison between different flowsheets and refolding schemes using a dimensionless economic objective. As expected from examination of the reaction kinetics, batch operation is the most inefficient mode. For the base process considered, the overall cost of fed-batch and continuous refolding is virtually identical (less than half that of the batch process). Reactor selection and optimization of refolding using overall economics are demonstrated to be vitally important.
DOI: 10.1002/aic.690430819
Appears in Collections:Aurora harvest 6
Chemical Engineering publications

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