Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/76211
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Djoko, K. | - |
dc.contributor.author | Franiek, J. | - |
dc.contributor.author | Edwards, J. | - |
dc.contributor.author | Falsetta, M. | - |
dc.contributor.author | Kidd, S. | - |
dc.contributor.author | Potter, A. | - |
dc.contributor.author | Chen, N. | - |
dc.contributor.author | Apicella, M. | - |
dc.contributor.author | Jennings, M. | - |
dc.contributor.author | McEwan, A. | - |
dc.contributor.editor | Weiser, J.N. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Infection and Immunity, 2012; 80(3):1065-1071 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | http://hdl.handle.net/2440/76211 | - |
dc.description.abstract | NGO0579 is annotated copA in the Neisseria gonorrhoeae chromosome, suggesting that it encodes a cation-transporting ATPase specific for copper ions. Compared to wild-type cells, a copA mutant was more sensitive to killing by copper ions but not to other transition metals. The mutant also accumulated a greater amount of copper, consistent with the predicted role of CopA as a copper efflux pump. The copA mutant showed a reduced ability to invade and survive within human cervical epithelial cells, although its ability to form a biofilm on the surface of these cells was not significantly different from that of the wild type. In the presence of copper, the copA mutant exhibited increased sensitivity to killing by nitrite or nitric oxide. Therefore, we concluded that copper ion efflux catalyzed by CopA is linked to the nitrosative stress defense system of Neisseria gonorrhoeae. These observations suggest that copper may exert its effects as an antibacterial agent in the innate immune system via an interaction with reactive nitrogen species. | - |
dc.description.statementofresponsibility | Karrera Y. Djoko, Jessica A. Franiek, Jennifer L. Edwards, Megan L. Falsetta, Stephen P. Kidd, Adam J. Potter, Nathan H. Chen, Michael A. Apicella, Michael P. Jennings and Alastair G. McEwan | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.rights | Copyright © 2012, American Society for Microbiology. All Rights Reserved. | - |
dc.source.uri | http://dx.doi.org/10.1128/iai.06163-11 | - |
dc.subject | Cells, Cultured | - |
dc.subject | Epithelial Cells | - |
dc.subject | Humans | - |
dc.subject | Neisseria gonorrhoeae | - |
dc.subject | Nitrites | - |
dc.subject | Copper | - |
dc.subject | Nitric Oxide | - |
dc.subject | Bacterial Proteins | - |
dc.subject | Virulence Factors | - |
dc.subject | Virulence | - |
dc.subject | Gene Deletion | - |
dc.subject | Microbial Viability | - |
dc.subject | Stress, Physiological | - |
dc.title | Phenotypic characterization of a copA mutant of Neisseria gonorrhoeae identifies a link between copper and nitrosative stress | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/IAI.06163-11 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP0986578 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/DP0986578 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kidd, S. [0000-0002-2118-1651] | - |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.